The Mechanism And Protection Role Of Diazoxide Postconditioning In Rat Liver

objective: To study the protection role of diazoxide postconditioningin rat liver. Methods: The model of70％hepatic ischemia reperfusionwas constructed in SD male rat．Twenty SD rats were randomly dividedinto four groups：sham group （5rats），ischemia-reperfusion group （I/Rgroup，5rats），diazoxide postconditioning group（DIPO group，5rats）．and blocker group（5-HD+DIPO group，5rats）. ischemia reper-fusion group (n=5, IR group), In DIPO group，rats were treated as IRgroup，but at the onset of reperfusion，reflow was initiated with dia-zoxidefor10min.In blockers group (5-HD+DIPO group), joining block er5-HDand diazoxide before restore blood flow, livers were subjected to120minof reperfusion,and compared to each change of serum ALT and AST, pro-tein immune imprinting method (Western blot) detected expression levelsof pkc-ε,Cyt-c,caspase-3and Bcl-2in liver tissue.compared with the Sgroup, three groups of ALT, AST content were increased significantly (P<0.05). compared with I/R, ALTand AST in DIPO group significantlywere lowered (P <0.05), the expression levels of pkc-ε and Bcl-2wereincreased significantly (P <0.05), the expression levels of Cyt-c and cas -pase-3were significantly reduced. It was obviously blocking the pro-tection role of postconditioning in5-HD+DIPO group. Conclusion:diazoxide-postconditioning can significantly reduce ischemia-reperfusioninjury in rat liver, which can promote the expression levels of pkc-εandBcl-2, and inhibit the protein expression apoptotic pathway by decreasingthe expression levels of Cyt-c and caspase-3, and protect the liver.