Differential Proteomics Analysis Of Endometriosis In Blood Stasis Syndrome

Background:Endometriosis (EMT) is a frequently-occurring disease in women of childbearing age. It imposes a dramatic impact on women reproductive health and quality of life. Syndrome is the comprehensive performance of etiology, location, quality and trend of diseases in a certain phase, and it’s the central conception of theoretic research of traditional Chinese Medicine (TCM). Therefore, to investigate the innate character and to reveal the scientific essence of syndromes are the crucial point of modernization of TCM theories. Furthermore, the breakthrough of biological research about syndromes might confirm the clarification of its scientific essence. Blood stasis syndrome is a common pattern in various clinic diseases and is the key pattern in EMT. In previous research, the differences on histological morphology and apoptosis associated factors between ectopic and reigning endometrium were revealed, that applied a foundation for further study on specific markers among EMT syndromes by proteomics. It is predominantly useful for EMT’s accurate diagnosis and effective management when the profound and systemic study on syndrome of blood stasis of EMT is accomplished.Objective:①To separate and identify the global protein by proteomics from EMT ectopic and reigning endometrium in3syndromes including blood stasis syndrome, qi stagnation and blood stasis syndrome as well as kidney deficiency and blood stasis syndrome, and to reveal the specific protein for EMT syndrome.②To detect the innate characters of the three syndromes of EMT, to investigate the molecular biological basis for syndrome differentiation of blood stasis of EMT, to establish the system of microscopic identification and diagnosis for EMT protein, to discover the transmission principles of EMT syndromes and their targets, and to search the meaningful molecular marker and genetic associated targets. Methods:Tissues from ectopic and reigning endometrium in proliferative phase in EMT patients were extracted, including6of blood stasis syndrome,6of qi stagnation and blood stasis syndrome, as well as6of kidney deficiency and blood stasis syndrome, and6specimens of normal endometrium in proliferative phase were extracted. Then each two specimens in all syndromes were randomly and isometricly mixed, consequently3groups of specimens were achieved to extract the total protein. Finally3groups’spectrums of running gel were accomplished by two-dimensional electrophoresis, and the different points were measured by MALDI-TOF-TOF/MS and protein database.Results:In blood stasis syndrome, two differential proteins from reigning endomutrium were obtained, while one from ectopic endometrium was obtained. In kidney deficiency and blood stasis syndrome, three differential proteins from reigning endomutrium were obtained, while three from ectopic endometrium were obtained. In qi stagnation and blood stasis syndrome, three differential proteins from reigning endomutrium were obtained, while four from ectopic endometrium were obtained. The annexin was highly expressed in both reigning and ectopic endometrium of kidney deficiency and blood stasis syndrome. The myoglobulin light chain3was highly expressed in both reigning and ectopic endometrium of qi stagnation and blood stasis syndrome.Conclusion:There are differential protein expressions among EMT three syndromes, which might be the inner origin of syndrome characters, and these differential proteins might be as the candidate biomarkers for pathogenesis of EMT various syndromes.