Research Painful Diabetic Peripheral Neuropathy Treatment Efficacy Mechanism Qi Blood Circulation

Objective:To investigate the effect of Mudan granule (Tangmoning granule) on streptozotocin (STZ)induced painful peripheral diabetic neuropathy rats serum pain substance fivehydroxytryptamine (5-HT), β-endorphin (β-EP), effect of histamine, bradykinin; and thevoltage gated sodium sural nerve in ion channel1.7,1.8,1.9(Nav1.7, Nav1.8, Nav1.9),vanilloid receptor1(TRPV1) mRNA expressioMethod：By intraperitoneal injection of streptozotocin model of painful diabetic peripheralneuropathy in rats given orally, Mudan granule, with phenytoin sodium as positive controlgroup, and normal control group and model control group, enkephalin pain rats serum3W,5W,9W weeks of painful diabetic peripheral neuropathy in five., β-were observed before andafter treatment (β-EP), histamine, bradykinin content changes; and using the method ofRT-PCR test before and after the treatment of the sural nerve in Nav rats of painful diabeticperipheral neuropathy in1.7, Nav1.8, Nav1.9, TRPV1mRNA expressioResult：The model group after making the mold3W,5W,9W in serum5-HT, histamine,bradykinin levels were significantly higher than that in the control group, there wasstatistically significant difference (P<0.01), and β-EP were significantly lower than those inthe blank control group, there were significant differences (P<0.01). Compared with themodel group in3W,5W,9W three time points of Mudan granule group and phenytoin serumhistamine, bradykinin levels were significantly reduced, there is significant difference(P<0.01); the serum5-HT in section3W of Mudan granule group compared with the modelgroup decreased significantly, there was statistically significant difference (P<0.05),phenytoin group and compared with model group decreased significantly, there wasstatistically significant difference (P<0.01); in5W,9W Mudan granule group and phenytoingroup and model group decreased, there was statistically significant difference (P<0.01);3W,5W,9W of Mudan granule group and phenytoin serum β-EP level was increased significantlyas compared with the model group, there is statistical differences (P<0.01). Compared withthe Mudan granule group:3W,5W,9W in5-HT group was significantly higher than that ofphenytoin sodium in serum of Mudan granule group, there was statistically significantdifference (P<0.05); histamine level phenytoin group was significantly higher than that ofMudan granule group, there was statistically significant difference (P<0.01); bradykinin levelswere elevated but no statistical difference (P>0.05); inside enkephalin levels were decreasedbut there was no statistical difference (P>0.05Expression of3W of model group,5W,9W Nav1.7, Nav1.8, Nav1.9, TRPV1assumes the trend of escalation, phenytoin group can downregulate the expression of Nav1.7, Nav1.8,Nav1.9, TRPV1,3W,5W, and9W decreased. Mudan granule group and phenytoin groupshowed the same change trend.Knottheory:1the successful model of evoked painful peripheral neuropathy in diabetic rat model, thismodel provides reference for similar model.2mechanisms of Mudan granule in the treatment of painful diabetic peripheral neuropathymay be associated with a decrease in expression of serotonin, histamine, pain in five ofbradykinin induced by serum levels of analgesic substances, increased endorphin levelsexpression.3.Inhibition of3of Mudan Granule on painful diabetic peripheral neuropathy in rats ofvoltage-gated sodium channels1.7,1.8,1.9channel, down-regulating expression, may bethe mechanism of improvement of painful diabetic peripheral neuropathysymptoms of.4of Mudan granule can reduce painful diabetic peripheral neuropathy vanilloid receptor1(TRPV1) expression, may be the mechanism of improving the painful diabetic peripheralneuropathy symptoms of...