MiR-181a Expression Vectors To Verify Its Influence On The Biological Behavior Of Pancreatic Cancer Cells

Background:Pancreatic cancer is a kind of malignant tumor with poor survival and high mortality that is difficult for diagnosis and treatment, for that novel tumor bio-markers and effective therapeutic strateges are urgently needed. MicroRNAs (miRNAs) are small RNAs with a length range between20-24nucleotides which participate life progress as negative post-transcriptional regulators. MiRNAs may become the new markers and therapeutic targets according to its abnormality in the expression patterns among the different cancer tissues. The higher expression level of miR-181a in pancreatic adenocarcinoma relative to chronic pancreatitis and normal pancreas may reveal the role of this miRNA in pancreatic carcinogenesis to some extent.Objective:Evaluate the effect of ectopic miR-181a expression on biological behavior of pancreatic cancer cell.Method:Transfect the recombinant vector with pre-miR-181a sequence and blank control vector into the pancreatic cancer cell MIAPaCa-2, respectively. Confirm the efficiency of the instant expression using real-time PCR and luciferase assay system. Compare the difference of cell growth, cell cycles, apoptosis, migration and invasion between the groups.Result:The real-time PCR and luciferase assay demonstrated successful construction of miR-181a overexpression model in vitro by the recombinant vector transfection. Overexression of miR-181a enhanced the invasion of the pancreatic cancer cell MIAPaCa-2. While there were no significant effects on cell growth, cell cycle, apoptosis and migration by ectopic expression of miR-181a.Conclusion:The overexpression of miR-181a could promote invasion of the pancreatic cancer cells by the transfection of recombinant vector.