Evaluation Of Sarcoidosis Diagnosis:Proteomics And Clinical Comprehensive Diagnosis

Objective:To Evaluate the function of proteomics in the diagnosis and differential diagnosis of Sarcoidosis, and thereafter help to find out new biomarkers in the future.Methods:Surface enhanced laser desorption ionization-time of flight-mass spectrometry was used to screen the serum of sarcoidosis and control patients. After statistical analysis and BPS cluster analysis, significant peaks related to the diagnosis of sarcoidosis, the differential diagnosis with tuberculosis and the activity of Sarcoidosis were found out, with their clinical effectiveness evaluated. The clinical information of Sarcoidosis and tuberculosis patients were analyzed and the effectiveness of usual methods such as SACE, PPD, TB-Elispot, CD4/CD8and characteristics in HRCT or bronchoscopy were evaluated, which were then compared to proteomic methods.Results:Seven nodes combined of M5797₇6, M11483₃, M1583₄8, M1296₄9, M1648₁2, M1607₃4and M1497₉0had the sensitivity and spectivity of94.87%, better than any methods used nowadays. Key node M11483₃was part of serum amyloid A, while M5797₇6was unkown. Key node M13752₀was much higher in sarcoidosis than tuberculosis, which would help for their differential diagnosis. Peaks M1038₀2, M1872₁9, M1967₅3and M9411₉9were significant between groups before and after corticosteroid therapy of sarcoidosis, which might related to the actitivity of disease. Combination of several clinical indexes was still needed for the differential diagnosis of sarcoidosis and tuberculosis.Conclusions:Proteomics could improve the clinical diagnosis and differential diagnosis of sarcoidosis, which will also help to find biomarkers in the future.