Application Of Stem Cells And Scaffold Technology Of The Treatment Of Diabetes

Induced-pluripotent stem cell (iPSC) is one of the most hot spots in cell replacement therapy of type1diabetes. There is no further study on the induction of iPSC to islet-like tissue with3D culture. This study explores the3D culture environment using PLGA scaffold combined with extracellular matrix (ECM) to induce iPSC into islet-like tissue.In part1of this study, iPSCs-1011were subcutaneously injected into immunodeficiency mice and observed the formation and growth of tumors which composed by the cells of three germ layers, which demonstrated multipotency of iPSCs-1011.In part2, iPSCs were cultured and induced in2D or3D culture, then be further identified by cell morphology, immunology and function tests. Many cells developed DTZ-positive vesicles in the cytoplasm. With immunohistochemistry staining, some of the cells showed positive for insulin, glucagon, and somatostatin. The glucose stimulating tests suggested there was a statistically significant elevation of insulin secretion between2D and3D culture (P<0.01). Under high glucose, insulin secretion of3D cultured cells had a statistically elevation compared with those of2D environment (P<0.05). The results demonstrated it is feasible to induce iPSCs into islet-like cells and scaffold-ECM culture environment has its unique advantages.In part3, we transplanted the islet-like cells intermuscularly into a diabetic rhesus model and then observed the increasing of blood insulin and decreasing of blood glucose after transplant. We also seek for the possibility of evaluating the implant function by PET-CT, SPECT etc.