The Significance Of The Expression Of CXCR4/CXCL12、CD44and CD147Marked Pituitary Adenomas In Their Invasive Behaviors

Objective Detect the expression levels of CXCR4, CXCL12, CD44, CD147in invasive and non-invasive pituitary adenomas, and comparatively analyze the relevance of these proteins and the invasiveness of pituitary adenoma and the probable mechanisms.Materials and Methods Collected the fresh specimens of surgical resection of35pituitary adenoms of our neurosurgery department from January to April,2009; made them to single cell suspension, and detected the expression of CXCR4, CD44, CD147by flow cytometry; at the same time, put the suspension under fluorescence microscope, observed the fluorescent-labeled cells and took photographed records; used the immunohistochemical methods to research the expression of CXCR4, CXCL12, CD44, CD147in the corresponding paraffin specimens of pituitary adenoma, and photographed them; used the SPSS17.0for statistical analysis, and took P<0.05as statistically significant standard.Results Based on clinical manifestations, imaging examinations, surgery and pathological diagnosis, the pituitary adenomas (35cases) were divided into invasive pituitary adenomas (21cases) and non-invasive pituitary adenomas (14cases) compairing Comparing the expression levels of these two groups, the P values of CXCR4, CD44and CD147were0.002,0.221and0.178respectively in the results of flow cytometry; the P values of CXCR4, CXCL12, CD44, CD147in immunohistochemistry method were0.04,0.01,0.516and0.855respectively; the expression levels of CXCR4/CXCL12in pituitary adenomas were higher than the levels in normal pituitary; fluorescence microscope showed that part of pituitary adenomas expressd the labelled tumor markers. The expression of CXCR4in pituitary adenoma which invaded buttom of sella turcica was significantly higher than that cavernous sinus invaded group(means were58.3%and23.6%, P<0.01). CXCR4/CXCL12, CD44, CD147had no significant relationship with patient gender, age, tumor size and function(P>0.05).Conclusion Flow cytometry is more accurate and sensitive in the detection of expression rate of tumor markers in tumor cells than immunohistochemistry.In invasive pituitary pituitary adenomas the expressions of CXCR4/CXCL12are significant increased, the expressions of CD44, CD147has no significant difference. CXCR4/CXCL12play an important role in the process of invasiveness of pituitary adenoma, possibly though the ERK1/2MAP kinase cascade and Pyk2activation and can be a new potential marker for the study of the abnormal biological behaviors of pituitary adenoma.