Impact Of Xiao Ke Wan On Human Vascular Endothelial Cells And Nitric Oxide

Objective:1. To investigate the effects of different parts of the traditional Chinese medicine XiaoKeWan on the proliferation of HUVEC;2. To investigate the effects of different parts of the traditional Chinese medicine XiaoKeWan on the production of NO of HUVEC;3. To investigate the effects of different parts of the traditional Chinese medicine XiaoKeWan on eNOS mRNA expression of HUVEC.This study was aimed at characterizing the response to XiaoKeWan of HUVEC, in terms of proliferation and production of NO, to clarify the function of XiaoKeWan on the endothelial cells and then discuss its therapeutic use from this point.Methods:1. HUVECs were cultured in the presence of different parts of the traditional Chinese medicine XiaoKeWan. XTT assay was applied to evaluate the cell growth.2. HUVECs were cultured in the presence of different parts of the traditional Chinese medicine XiaoKeWan. Then the total NO in the medium was measured with the Griess method.3. Realtime PCR was applied to measure the levels of eNOS mRNA expressing in the culture of different parts of the traditional Chinese medicine XiaoKeWan.Results:1. XiaoKeWan and its Chinese medicine part exhibited no obvious effect on the proliferative activity of HUVEC.2. XiaoKeWan, the Chinese medicine.part of XiaoKeWan, and glyburide all increased the production of NO.3. The Chinese medicine part of XiaoKeWan increased the expression of eNOS mRNA, but no significant differences.Conclusion:Our study discovered that XiaoKeWan and its Chinese medicine part exhibited no obvious effect on the proliferative activity of HUVEC; XiaoKeWan, the Chinese medicine part of XiaoKeWan, and glyburide all increased the production of NO; the Chinese medicine part of XiaoKeWan increased the expression of eNOS mRNA, but no significant differences.