Xiao Ke Wan Effects On Human Vascular Endothelial Cell Growth And Function

ObjectiveTo investigate the effect of a chinese triditional medicine, which is called "Xiaokewan", on the release of nitric oxide (NO) inculture endothelial cell from human umbilical vein。 And the effect of this medicine on the proliferation of human umbilical vein endothelial cells (HUVECs) was also studied。MethodsHUVECs, were isolated by collagenase digestion。 The3th generation of cultured HUVECs were used with the cell count of1×105/ml in each core The experiment consisted of4groups:(A) glibenclamide group;(B) full medicine of "Xiaokewan" group;(C) medicine of "xiaokewan" without group;(D) control group; and three different concentrations for each group were compared。For the study of effect of "Xiaokewan" on the proliferation of HUVECs,4groups were assigned randomly:(a) control group;(b) low concentration group;(c) moderate concentration group;(d) high concentration group。At day1、day3and day5count the cells in each core。Results"Xiaokewan" promoted the secretion of NO, and this effect was mainly due to the medicine without glibenclamide。High concentration of "Xiaokewan" inhibited the proliferation of HUVECs。ConclusionsFor each concentration," Xiaokewan" can promote the secretion of NO of HUVECs in vitro and high concentration of "Xiaokewan" inhibit the proliferation of HUVECs。...