Analysis Of The Electroencephalogram And Super Encephalofluctuograph Technology On"Rigid-Gentle Syndrome Differentiation"of Psychosomatic Diseases

Objective:To explore the material bases and the nature of’Rigid-Gentle Syndrome Differentiation’ of psychosomatic medicine from the aspects of cerebral cortex functions and brain neurotransmitters levels by the methods of electroencephalogram (EEG) and super encephalofluctuograph technology(SET).Methods:Syndromes are the core while diseases are the complement. The accuracy of Syndrome Differentiation has been verifying by the curative effects of fixed traditional Chinese medicine (TCM) prescription. Each case was summarized by curative effects according to efficacy standards of different diseases. Brain electrical activity mapping (BEAM)(linear analysis method of EEG) and SET (nonlinear analysis of EEG) were used as technical means to measure the cerebral cortex functions and brain neurotransmitters levels. Each case has been measured by BEAM and SET before treatment.100cases of the four syndromes of’Hyperactivity of heart-fire and liver-fire’,’Heart and liver yin deficiency’,’Stagnation of QI due to depression of the liver’and’Stagnation of liver-QI with deficiency of the spleen’ which belong to the system of’Rigid-Gentle Syndrome Differentiation’ were enrolled in this study, and in order the number of cases are20,32,27and21. In addition, the control group is of23healthy volunteers. The differences of cortical areas, brain wave rhythms and power values in BEAM and neurotransmitters levels in SET have been compared among the four syndromes groups and the control group. The data was analyzed by SPSS18.0and the statistical methods are descriptive analysis, One-way ANOVA, Independent-samples T-test, Paired-samples T-test, and Kruskal-Wallis H test, Chi-square test and so on. All statistical tests were two-sided (a=0.05).There is a significant difference if p valve is less than0.05or0.01.Results:(1) The regularities and specificities of four syndromes compared with control group in BEAM:①The three syndromes of’Hyperactivity of heart-fire and liver-fire’,’Stagnation of QI due to depression of the liver’and’Stagnation of liver-QI with deficiency of the spleen’ have roughly the same expressions in BEAM:the rising trend of slow wave power in the forebrain(P<0.05); the decreasing trend of α wave power in parietal-occipital region(P<0.01); the decreasing trend of β wave power in frontal pole(P<0.01), frontaland parietal-occipital region(P<0.05). The different expressions in BEAM of these three syndromes were that a wider range of slow wave rhythm of’Stagnation of liver-QI with deficiency of the spleen’ syndrome expanding to frontal and central region (P<0.05)beside frontal pole; a slower wave rhythm of ’Hyperactivity of heart-fire and liver-fire’syndrome including δ activity(P<0.05) besides0wave.②The specificity of the syndrome of’Heart and liver yin deficiency’in BEAM:the rising trend of slow wave power including0wave(P<0.05) and8wave(P<0.01) in twelve cortical areas except double temporal region which is much wider than other three syndromes; forward and generalized trend of a wave rhythm (P<0.01)and no suppression of the ascendancy of a wave rhythm in parietal-occipital region; the decreasing trend of β wave power in frontal pole and frontal region(P<0.05).(2)The regularities and specificities of four syndromes compared with control group in SET:①The reduced activity of Dopamine(DA) in right Prefrontal Cortex maybe the one of common material bases of’Rigid-Gentle Syndrome Differentiation’ theory in cerebral function changes(P<0.05).②Left Mid-temporal3region(T3) maybe the main cerebral region of’Rigid-Gentle Syndrome Differentiation’theory in cerebral function changes(P<0.05).③The co-operation of reduced activity of Excitatory Neurotransmitter (EXC) and increased activity of Inhibitory Neurotransmitter (INH) in T3maybe the one of material bases of’Rigid syndrome’in’Rigid-Gentle Syndrome Differentiation’ theory(P<0.05).④The reduced activity of EXC in brain maybe the one of common material bases of the three syndromes of’Hyperactivity of heart-fire and liver-fire’,’Heart and liver yin deficiency’and’Stagnation of QI due to depression of the liver’in cerebral function changes(P<0.05).⑤The co-operation of Acetylcholine (ACh) and Norepinephrine(NE), and5-Hydroxytryptamine(5-HT) and DA in T3maybe the one of material bases of’Heart and liver yin deficiency’ syndrome in cerebral function changes(P<0.05).⑥The abnormal activity of NE in left Prefrontal Cortex maybe the one of material bases of’Stagnation of QI due to depression of the liver’ syndrome in cerebral function changes.(P<0.05)⑦The increased activity of INH in T3maybe the one of material bases of’Stagnation of liver-QI with deficiency of the spleen’ syndrome in cerebral function changes(P<0.05).Conclusions:①The results of this study have partly revealed the regularities and specificities of four syndromes of’Rigid-Gentle Syndrome Differentiation’ theory in BEAM and SET. This study has preliminarily provided the evidences of the material bases and the nature of’Rigid-Gentle Syndrome Differentiation’ of psychosomatic medicine from the aspects of cerebral cortex functions and brain neurotransmitters levels.②At same time, the research mode of’Syndromes are the core while diseases are the complement’ has been established which will play a role of demonstration effect in the future study of’Rigid-Gentle Syndrome Differentiation’.③This study also could set up target indexes for diagnosis, effectiveness evaluation, screening effective drugs and their mechanisms of psychosomatic diseases from the aspects of cerebral cortex functions and brain neurotransmitters levels.④This study indicates that negative stimuli of psycho-social factors can affect cerebral function, which causes organic disorders or process of pathologic physiology. This study provides the brain electrophysiological evidence for the TCM psychosomatic medicine theory "If the mentality is normal, the organs will function normally. This is the fundamental principle to keep good health and a long life" and "If the mentality is abnormal, it is very dangerous to the twelve organs including itself." ⑤This study also provides modern scientific data for basic researches of TCM syndrome from the EEG signals.