| Objective Evaluate the expression of hTERT.UbE2D3, and UbE2C in cervical cancer tissue and analyze the role of these genes coding protein in the process of development and the correlation in cervical cancer, and the relationship between hTERT, UbE2D3,UbE2C and clinical pathological factors of cervical cancer, their effect on the prognosis of cervical cancer research, and explore the related mechanism of molecular pathology.Materials and Methods Clinical data of173cases of surgical tissue specimens from wax of patients with cervical cancer saved by the department of pathology of wuhan university zhongnan hospital between2002-2012were analyzed restrospectively. We use the immunohistochemical staining to detect the expression of hTERT, UbE2D3and UbE2C protein. We use SPSS17.0statistical software to do the statistical analysis, we use x2test and Fisher’s exact probability method to analyze different expression levels of hTERT, UbE2D3and UbE2C in different stages,classification,grade in cervical cancer tissue, hTERT, UbE2D3and UbE2C’s influence on the prognosis of patients with cervical cancer by Kaplan Meier-method and the Log rank test, the evaluation application of the correlation of the expression of hTERT and UbE2D3,UbE2C using Kendall’s tau-b rank correlation analysis, the factors affecting the prognosis of patients with cervical cancer using Cox multiple factors analysis, P<0.05for the difference was statistically significant.Results hTERT protein positive signals are mainly distributed in the nucleus, and UbE2D3are distributed both in the nucleus and cytoplasm. hTERT protein expression in cervical cancer tissue is not closely related to the patients age, pathological type, stage, grade, P>0.05, but is closely related to the cervical lymph node metastasis, which means positive expression rate of lymph node metastasis group hTERT was significantly higher than that of group without lymph node metastasis, P<0.05, at the same time UbE2D3protein expression has nothing to do with the patient age, pathology type, grade, P>0.05, but is associated with cervical cancer staging and lymph node metastasis,the later stage UbE2D3protein expression was lower than those of early stage early, at the same time, lymph node negative metastasis group UbE2D3expression rate was significantly higher than that of group lymph node metastasis, P<0.05). UbE2C protein positive signals were distributed in the nucleus and cytoplasm. UbE2C protein expression in cervical cancer tissue has nothing to do with patient’s age. pathology type. P>0.05, but its expression is closely related to the cervical cancer staging, grading and lymph node metastasis,which means the stage high group UbE2C protein expression are significantly higher than the low group, high degree of differentiation group UbE2C expression quantity is significantly lower than the low degree of differentiation, lymph node metastasis group UBE2C protein positive expression rate was significantly higher than that of group without lymph node metastasis, P<0.05). The expression of UbE2D3UbE2C and hTERT are associated with the prognosis of patients with cervical cancer.means that the lower the expression of UbE2D3is,the cervical cancer patients prognosis is the poorer, the higher the expression quantity of UbE2C and hTERT is,the poorer the prognosis is, the difference is statistically significant, P<0.05. The correlation between expression of hTERT and UbE2D3protein inCervical cancer tissue was statistically significant (fairly rk=0.330, P<0.001), and the expression relationship between UbE2C and hTERT protein in cervical cancer tissue were positively correlated,(fairly rk=0.483, P<0.001). At the same time, Cox multiple factor method analysis shows that protein expression levels of hTERT, UbE2D3,UbE2C, tumor grade and lymph node metastasis is the independent factors which affecting the prognosis of cervical cancer.Conclusions1,The excessive activation of hTERT in cells in the occurrence and development of cervical cancer plays an important role in the process. HTERT protein can be used as a cervical cancer diagnostic and prognostic molecular markers, were the independent factors influencing the prognosis of cervical cancer, high hTERT expression suggests poor prognosis of cervical cancer patients.2, UbE2D3is cervical cancer occur in the development of protective factors, and negatively correlated with the expression of hTERT. UbE2D3is the independent factors affecting the prognosis of cervical cancer, low UbE2D3expression suggests poor prognosis of cervical cancer patients.3, UbE2C development, with the incidence of cervical cancer staging classification and prognosis is closely related to UbE2C is not only involved in the initial stage of cervical cancer become, all at the same time also participated in the cancer process, UbE2C is positively related with the expression of hTERT, UbE2C is the independent factors affecting the prognosis of patients with cervical cancer, high UbE2C expression suggests poor prognosis of cervical cancer patients. |
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