The Correlativity Of Overexpression Of Bcl-2in Vascular Endothelium And Retina Angiogenesis

Part I The Correlativity of Overexpression of Bcl-2in Endothelial Cell and Retinal AngiogenesisObjective:to investigate the physiological role of overexpression of Bcl-2in vascular endothelium in the development of retinal vasculature and retinal neovascularization formation during oxygen-induced ischemic retinopathy (OIR).Method:We observed the retinal vasculature development of Bcl-2overexpressed in endothelial cells in retinal whole mount slides in different time points (P7, P14, P21, P42), then compare the nonperfusion area and neovascularization with wild type mice in ROP model.Results:The retinal vascular development did not impacted by the Bcl-2overexpression in endothelial cells. Overexpression of Bcl-2in endothelium can not prevent retinal nonperfusion area or neovascularization formation in ROP.Conclusions:overexpression of Bcl-2in endothelium have no effect on the retinal vascular development and retinal neovascularization. Part Ⅱ The Impact of The Change of Bcl-2Content in Endothelial Cells and The Formation of Choroidal NeovascularizationObjectives:To assess the impact of deficiency and overexpression of Bcl-2in endothelial cells on choroidal neovascularization (CNV).Methods:Three laser burns were placed in each eye in mice to induce CNV. For quantitative measurements of neovascularization, intercellular adhesion molecule2staining was performed at14days postlaser of the choroidal-sclera flat mounts. The CNV area was measured by intercellular adhesion molecule2staining and use of ImageJ softwareResults:Deficiency of Bcl-2in endothelial cells exhibited significantly smaller areas of neovascularization on choroidal flat mounts compared with WT mice,however the overexpression of Bcl-2in endothelial cells have the same areas of neovascularization as WT mice.Conclusions:Deficiency of Bcl-2in endothelial cells contributes to reduced choroidal neovascularization.