Expressions Of ABCAI、NF-КB On Acupuncture Treating ApoE^-/- Mice

Objective: to observe the electrical Lee in Apo E- /- mice, aortic sinus ABCAI, NF- B К expression.To explore the curative mechanism of action of the AS pathological changes, so AS to provide clinical treatment of acupuncture and moxibustion AS some basis for content.Methods: the 30 experimental(Apo E- /-) mice were randomly divided into three groups, and marked with model group, drug and electric acupuncture group.Electric acupuncture group: choose three mile "after", "pass", "three Yin", "door god" acupuncture points around, and some local disinfection operation, in the process, can undertake certain after acupuncture, then to asaps cupping therapy apparatus connected, in the process output lines connected to the AB, A on mice’s "off" and "god", and through to the B is mice "after three mile" and "three vaginal intercourse".In the process, including the use of dilatational wave is 2/15 hz, and ten minutes of acupuncture, current intensity of 0.3 ma, every time just for unilateral acupuncture, and left, two days for a treatment, a total of seventy-seven days.Drug groups: the experimental animal the ultrapure water allocation of simvastatin lavage treatment(the amount of 40 mg/kg. D), once per day, every day and will be fixed on the beam of mice, each fixed 10 min;Model group: ultrapure water for experimental animals lavage treatment(the amount is 0.1 ml/g), once per day, every day and will be fixed on the beam of mice, each fixed 10 min;Results:(1) in the fatty group, aortic sinus plaques in the nf-kappa B are mainly distributed in the fibrous cap.Large amounts of the nf-kappa B positive particles distributed in lining and lining, the film also has a small amount of expression, at the same time, also have sporadic expression in the aortic valve.Group and fatty plaques contain cholesterol crystals as well as a wide range of nuclear and accompanied by a large number of apoptotic cells necrosis and death.The nf-kappa B positive particles in drug group plaques high fat group decreased significantly, and also relative to reduce cholesterol crystals and necrotic cells;In acupuncture group with a small amount of the nf-kappa B expression on the fibrous cap, but compared with high-fat group significantly reduce the amount of its expression, further observation found its expression in aortic volume also decreased significantly.(2) acupuncture group of aortic sinus intimal plaque in a large number of cell apoptosis and cell death phenomenon, at the same time accompanied by the appearance of cholesterol crystals.In the drug group, ABCA1 protein expression in the fibrous cap the amount significantly more, relatively stable.Under a microscope observation of the drug group related biopsy further found that lipid deposition and a lot of foam cells contained in endothelial cells and in mesenchymal cells of ABCA1 positive brown granules is very much.This represents the endothelial cells to absorb lipids can for the expression of ABCA1 play a stimulating role.Drug group of primary endothelial cells and aortic sinus were only a small amount of brown granules were positive.(3) model group and drug group contained in the plaque is a lot of gruel necrotic core and cholesterol crystals, and also contains a lot of dead cells.Microscope found that patches of very thin fibrous cap in model group, and its elastic fiber almost disappear.Drug group appear the phenomenon of plaque rupture, and compared with acupuncture group, the elastic fibers in the drug group is significantly reduced, in the observation of the tissue section after acupuncture therapy found that compared with the drug group and model group, plaques in the acupuncture group thicker fibrous cap, and some still has not been formed, it shows that the plaque stability, and compared with drug group and model group, acupuncture group of lipid plaque area is relatively less in the group.And further observation found that acupuncture group of plaque mainly contain a large number of foam cells, this time a lot of foam cells showed that the plaques are still in the phase gradient, a lot of gruel necrosis and cholesterol crystals show that the foam cells are gradually dying, says it is atherosclerotic plaques phase state.That is to say, acupuncture treatment, can have certain protective effect to vascular intima, and for the development of plaque inhibition effect, relative to drug treatment curative effect will be better.Conclusion: electric acupuncture treatment can for vascular intima play a protective role, and for smooth muscle cell migration and appreciation have certain inhibition effect, avoid the generation of inflammation.Electric acupuncture can make the Apo E- /- mice improved lipid metabolism of the body, and in the process for the development of plaque inhibition effect, make the neointimal protected, it is showed that the lipid metabolic disorder AS a mechanism may lead to the occurrence of the AS.