| Objective To study the relationship between CYP11B2-344T/C geneticpolymorphisms and susceptibility to Coronary Heart Disease in Tangshan Area. Methods A case-control study was carried out. 400 hospitalized patients was selected in the department of cardiology of Hebei United University Affiliated Hospital,Tangshan,Hebei province. The coronary lesions wered evaluated by quantitative coronary angiography(QCA) method.The selected population were divided into Coronary heart disease group(CHD)and control group,acute myocardial infarction group and angina group, single-vessel group and multi-vessel group, high-points Gensini group and low-points Gensini group based on the QCA. The gender, age, history of smoking, drinking, hypertension, diabetes mellitus, family situation and drug use were recorded.The biochemical analyzer of LDLC(low density lipoprotein), HDLC(high density lipoprotein), APOA(apolipoprotein A),TC(total cholesterol), TG(triglycerid), APOB( load lipoprotein B), Lpa(polipoprotein(a) were measured using automatic biochemical analyzer. CYP11B2-344 T / C gene polymorphism were detected by Polymerase chain reaction- restriction fragment length polymorphism(PCR-PFLP). All data were inputed into the database of Excel 2000 and analysed statistically by spss21.0, the allele frequencies wered calculated on the gene counting method, Hardy-Weinberg equilibrium of gene locus were detected by methods of fitness of frequency distribution, Measurement data were analysed by t- test and ANOVA,the count data were analysed by chi-square test. The interaction among the mutiple factors wered analysed by Logistic regression analysis.Result: 1. The age distribution of male CHD patients is maily between 50-70 years old( 64.2%),there is no difference in the age distribution of male CHD patients.The age distribution of female CHD patients is maily between 60-70 years old( 51.3%),there are difference in the age distribution of female CHD patients, 50-60 years old accounted for 23.7%, 60-70 years old accounted for 51.3% in the CHD group, 50-60 years old accounted for 47.9%, 60-70 years old accounted for 31% in the control group. The age distribution of male AMI is mainly age 50-70 years old(55%), female AMI is mainly male age 60-70 years old(55%), there is no difference in the age and sex distribution of AMI patients. 2.The number of male patients was higher(69.7% vs 51.6%)in CHD group compared with the control group.There was no difference with age and gender between AMI and angia group.3. The patients numbers having smoking history, hypertension and diabetes history is higer in the CHD group than the control group(49.6% vs 34.4%, 61.9 vs 46.3,27% vs 12.5%), Serum Lpa level is higer in CHD group than the control group(280.27 ± 279.92 vs 181.12 ± 169.47). the level of serum of APOA and HDL is lower in CHD patient than the control group(1.17 ± 0.24 vs 1.31 ± 0.25; 1.20 ±0.28 vs 1.34 ± 0.32). Total cholesterol, triglycerides, LDL-C, APOB have no differences between the two groups(4.82 ± 1.2 vs 4.89 ± 1.08; 1.76 ± 1.29 vs 1.92 ± 1.46; 2.98 ± 0.86 vs 2.92 ± 0.81; 0.91 ± 0.25 vs 0.89 ± 0.24). Multiple logistic analysis showed that age(OR1.031), male(OR2.378), hypertension(OR1.810), diabetes(OR2.760), smoking(OR1.905), high plasma LPa(OR2.305)is coronary heart disease in dependent risk factors, Apo A is a protective factor for coronary artery disease(OR0.214).In the AMI group, the level of TC, TG, LDL-C, LPa, APOB, HDLC, APOA respectively were 4.92 ± 1.44,1.56 ± 0.93,3.08 ± 1.11,346.5 ± 308.18,0.91 ± 0.25,1.20 ± 0.29,1.14 ± 0.26; In the angina group were respectively 4.80 ± 1.08,1.84 ± 1.42,2.94 ± 0.76,251.05 ± 260.82,0.91 ± 0.24,1.20 ± 0.27,1.18 ± 0.24.The were no significant difference in the clinical and Biochemical indicators between the AMI and angina group. 4.All included in the study,the three genotypes of TT, TC, CC were respectively48.5%, 42.0%, 9.5%, T allele is 69.5%, C allele is 30.5%. The distribution of genotypes and alleles have no difference in age and gender. Compared CHD group to control group, the distribution of three genotype TT, TC, CC was no difference. But diabates-stratified analysis found that TC+CC genotype in non-diabates patients was significantly higher(56.1% vs 43.8%) than the control group.(OR is 1.636) in the CHD group than the control groups, The genotype and allele frequencies were no significant difference in the AMI and angian groups, single-vessel and multi-vessel group, high-points Gensini and low-points Gensini group. In order to observe whether genotype have effects on CHD occurrence through risk factors,we futher compared the relationship between genotype and risk factors, the results found frequence of TC+CC genotype is higher in smoker than no- smokers(48.8% vs 38.7, P = 0.044, OR=1.512), the results showed that C allele of CYP11B2 is associated with cigarette smoking.Conclusion(1)The proportion of smokers with CC+TC of CYP1B2-344T/C was higher than TT genotype.(2)The risk of coronary heart disease with C allele of CYP11B2-344T/C was higher than that of TT genotype in non-diabetes patients.(3)The levels of serum Lpa were independent risk factor for coronary disease.(4) The frequencies of TT,TC, CC were 45.2%, 45.2% and 9.6%, the allele frequencies of C and T were 32.7% and 67.8 for CHD patients in Tangshan area.(5) Male, smoking, hypertension, diabetes, Lpa were indepent risk factors for coronary heart disease, APOA was protective factor for CHD. |
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