| Objective Probe the pathogenesis by traditional Chinese medicine and western medicine through theoretical and practical research on obesity,provide theoretical guidance for the clinical application of the theory of traditional Chinese medicine treatment of obesity, through the high cholesterol diet-induced obesity animal model to investigate the WSJPHT Decoction on obesity effect of disease in rats model, from the molecular level and pharmacology research the mechanism of action on obesity by warming the kidney and invigorating the spleen, so as to provide a theoretical basis for WSJPHT Decoction and resolving clinical treatment of obesity.Method This study was conducted from theoretical and experimental research on two aspects. Theoretical Studies:From diagnosis, classification and etiology of obesity pathogenesis, and diagnosis and treatment aspects of the system are discussed, and from four aspects principle-method-recipe-medicines for warming the kidney and invigorating the spleen to treat obesity mechanism discussed theory.Experimental studies: 60 healthy male SD rats weighing 100-150 g were randomly divided into 2 groups, one for the normal control group, 10 rats given normal diet, free feeding water; the remaining 50 high fat diet freely feeding water. Measurement of body weight twice a week, select body mass feeding after 4 weeks beyond the normal average body mass of 40 to 15% and Lee’s index increased by more than 1.5% as successful model in rats. The 40 successful model rats were randomly divided into four groups, namely, model group, low dose group, middle dose group and high dose groups of 10. Normal group and model group were given normal saline 1 ml / 100g·d orally; low, medium and high dose groups of rats were given low, medium and high doses of warm the kidney and invigorate the spleen party 1ml / 100g·d ig. Continue with the normal group fed normal diet, the other four groups continue to be high-fat diet, body weight was measured weekly during the experiment twice, and adjust the dose according to body weight, for 10 consecutive weeks. Observe the changes of rats in general and quality, the use of automatic biochemical analyzer to detect the serum lipid and liver function. Using HE staining pathological changes in liver tissue of rats in each group and adipose tissue. By RT-PCR was used to observe the impact of WSJPHT Decoction liver SOCS3 gene expression were observed on rats Ghrelin levels in adipose tissue immunohistochemistry.Result 1) Changes in body weight of rats in each group: For square low, medium and high dose could significantly increase the control of body weight in obese rats, body weight was significantlyslowed the rate of increase, compared with the model group statistically significant(P<0.01), and the middle dose group and high dose group was significantly better than low-dose group and was statistically significant(P<0.01), and the difference between the high dose group was not statistically significant. 2) Each group adipose tissue and liver histopathological changes: normal hepatocyte morphology normal arrangement, lobular rules, uniform cytoplasm; model group appear varying degrees of diffuse hepatic steatosis, liver cell volume increases found in the cytoplasm varying amounts and sizes of circular lipid droplets or fat vacuoles, the nucleus is pushed to the periphery; low dose group steatosis hepatocyte model group fairly; high dose group steatosis hepatocytes compared with the model group decreased significantly, showing a small amount of fat vacuoles. Compared with normal rats, rat adipocytes model group was significantly increased, per visual field significantly reduce the number of fat cells; low dose group and the model group rat adipose cells rather; in rat adipocytes compared with model high dose group significantly reduced the number of fat cells pervisual field was significantly increased, and the change in dose group more significant. 3) Rats liver function comparison: Compared with normal group, model group and low, medium, ALT high-dose group, AST were significantly higher(P<0.01, P<0.05); compared with the model group, WSJPHT Decoction square low, medium and high dose could significantly reduce ALT, AST level(P<0.01), and the middle and high dose group was significantly better thanlow-dose group(P<0.01), high-dose group decrease AST levels and significantly better than the middle dose group(P<0.01), but in reducing ALT levels had no significant difference(P>0.05). 4) Comparison of serum lipid in each group: Compared with normal group, model group and low, medium, TC high-dose group, TG, LDL-C were significantly higher(P<0.05), HDL-C decreased significantly(P<0.05, prompted obese rats obvious dyslipidemia compared with the model group, low WSJPHT Decoction phlegm side, middle and high dose group could significantly decreased TC, TG and LDL-C(P<0.01), increased HDL-C(P<0.01), and the comparison between the three groups also have significant differences(P<0.01). 5) Each group rat liver tissue SOCS3 gene expression: significantly increased(P<0.01) the amount of gene expression in rat liver tissue in model group, after treated by warming the kidney and invigorating the spleen, compared with the model group, drug treatment group gene expression was significantly decreased(P<0.01), in which the high dose group and low dose group compared to significant differences(P<0.01), compared with the normal group had no significant difference(P>0.05). 6) Ghrelin gene expression in adipose tissue of rats: Ghrelin gene expression of adipose tissue in the model group increased significantly(P<0.05),by warming the kidney and invigorating the spleen, compared with the model group, drug treatment group Ghrelin gene expression was significantly decreased(P<0.01), in which high dose group showed no significant difference(P>0.05), but compared with thelow-dose group had significant differenc(P<0.01) rats Ghrelin liver tissue immunohistochemistry results: light microscopy of liver cell nuclei were brown or tan as Ghrelin positive cells, negative nuclei blue or blue-violet. Observe the rat hepatocytes: liver cells of rats in the model group Ghrelin positive cells was significantly increased compared with the normal group, was significantly reduced by rat hepatocytes WSJPHT Decoction drug treatment Ghrelin positive cells compared with model group, in the high dose group was more apparent. IPP The results also showed that: Compared with normal group, model group, low positive expression rates and high dose groups Ghrelin increased significantly(P<0.05), after treatment, low, rat liver, high dose group Ghrelin the positive expression rate was significantly decreased compared with the model group(P<0.01).Conclusion Theoretical Studies: Obesity pathogenesis based virtual reality, performance-oriented spleen and kidney deficiency as the origin, phlegm and blood stasis as the standard. WSJPHT Decoction reflects obesity diagnosis and treatment, for the application of the treatment of obesity provides a theoretical basis. Experimental Studies: 1) WSJPHT Decoction party in control obese rats weight gain, reduce liver damage, reduce abdominal fat, lower serum cholesterol and triglycerides and improved liver steatosis aspects have a good effect. 2) WSJPHT Decoction before regulation appetite, increaseenergy consumption, inhibit fat synthesis, improve glucose and lipid metabolism, reduce obesity may be related to obesity by regulating the level of SOCS3 rat model to achieve. 3) WSJPHT Decoction only regulate the expression of Ghrelin gene in rat model of obesity, and regulate expression of these genes may not only directly affect the appetite and diet, increase energy consumption efficiency, but also by adjusting the adipokines leptin, reach the purpose of the treatment of obesity. |
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