Effect And Mechanism Of MiR-517a-3p And MiR-610 On Regulation Of Lung Cancer Cell Proliferation And Invasion

Aim: Aberrant expression of micro RNAs(mi RNAs) results in alterations of various biological processes(e.g., cell cycle, cell differentiation, and apoptosis) and cell transformation. Altered mi RNAs expression was associated with lung carcinogenesis and tumor progression. This study aimed to investigate the function and underlying molecular events of mi R-517a-3p and mi R-610 on regulation of lung cancer cell proliferation and invasion.Method: Transfected both mi R-517a-3p and mi R-610 mimics or inhibitors into 95 D and 95 C cells respectively, the effects of mi R-517a-3p and mi R-610 on lung cancer cell proliferation, migration, and invasion were detected. Bioinformatics software forecasted potential target genes of mi R-517a-3p and mi R-610. Dual luciferase reporter gene system and western blot verified whether mi R-517a-3p regulates FOXJ3 expression directly,as in the case with mi R-610 regulates GJA3 expression.Result: mi R-517a-3p and mi R-610 were both differentially expressed in lung cancer 95 D and 95 C cell lines that have different metastatic potential. Manipulation of mi R-517a-3p and mi R-610 expression changed lung cancer cell proliferation, migration and invasion capacity. mi R-517a-3p directly regulated FOXJ3 expression by binding to FOXJ3 promoter, with mi R-610 regulating GJA3 expression by binding to GJA3 promoter.Conclusion: This study demonstrated that mi R-517a-3p promoted lung cancer cell proliferation and invasion by targeting of FOXJ3 expression, on the contrary, mi R-610 inhibited lung cancer cell proliferation and invasion by targeting of GJA3 expression.