RP Lncrna Expression Signature And Qiao Minng Xue Arranfed Acupuncture And Tongqiao Mingmu NO.Ⅲ Intervention Treatment Of RP

Objective:Efficacy Qiao ming xue Arranged Acupuncture and Chinese herbal medicine prescriptions Tongqiao Mingmu No. Ⅲ of retinitis pigmentosa (RP),Research on acupuncture and medicine intervention on RP retinas and Qiao ming xue Arranged Acupuncturethe superiority. Examined the effects of vision, vision changes and OCT retinal nerve fiber layer thickness, study Acupuncture and Medicine on RP visual function and its possible mechanism.Methods:Select diagnostic inclusion criteria, RP 119 patients with a total of 60 cases were randomly divided into two treatment groups:Qiao ming xue ArrangedAcupuncture and Tongqiao Mingmu No.Ⅲ, Group: Other Qiao ming xue Arranged Acupuncturepoints of acupuncture except.Visual acuity, visual field and on the retinal nerve fiber layer thickness to carry out intra-group before and after treatment and comparison groups. We examined the effects before and after treatment visual acuity, visual field and OCT.Qiao ming xue Arranged Acupuncture and Tongqiao Mingmu No.Ⅲ the therapeutic effect of RP.Result:Before and after treatment in the experimental group compared with the control group vision:the treatment group was 61.67%, the control group was 44.07%, the difference was statistically significant (P<0.05).2. Before and after the experimental treatment group compared with the control group, the mean visual field defect:before treatment the mean visual field defect is 17.79+4.07, after treatment 11.03+4.824; the control group before treatment 16.39±5.34, after treatment 12.05± 4.889. Statistical analysis proved the difference was statistically significant (P<0.05);3.Before and after treatment OCT experimental group and control group, the mean thickness of the disc periphery comparison:The total effective rate was 58.33% in the control group, the total effective rate was 54.24%. There are differences between two sets of data, the test was not statistically significant (p> 0.05).Conclusion:Qiao ming xue Arranged Acupuncture and Tongqiao Mingmu No.ⅢRP therapy can improve vision, broaden horizons; show after short-term treatment can improve visual function, no obvious morphological changes.Objective:To explore the expression through RP and other eye patients LncRNA characteristic spectrum, and explore the potential regulatory pathway genes in peripheral blood LncRNA the RP, the RP for early diagnosis of disease provide new ideas.method:1. SampleFrom the First Affiliated Hospital of Heilongjiang University of Chinese Medicine Ophthalmology ward three cases of RP in patients with peripheral blood sample to the experimental group and three normal subjects as controls. The company uses Arraystar human LncRNA chip V3.0. The use of microarray transcription LncRNA 30586 and 26109 LncRNA protein-coding genes, and the present study tested samples of human protein-coding transcripts and high-throughput differential expression analysis.2. RNA labeling and hybridization arraysApplication of phenol-chloroform extraction of total RNA. Then, each sample was reverse transcribed cRNA by RNeasy Mini Kit for purification and concentration determination. Application of mRNA microarray hybridization labeled cRNA and then washed, fixed and Agilent DNA Microarray Scanner (Part No. G2505C) scan.3.Data AnalysisAgilent Software 11.0.1.1 array image analysis obtained by dividing the number of bits and standardized data processing GeneSpring GX V12.1 package. Will be selected for further analysis of the data after the standardization of raw data LncRNAs and mRNA. Thus having screened differentially expressed. Through Pathway analysis and GO analysis, LncRNAs/mRNA determine biological pathways in the regulation.Results:In this study, samples of human protein-coding transcripts LncRNA and globally cluster enrichment analysis and other analysis and found that the expression of RP LncRNA mainly related disease has been LncRNA, mRNA gene interactions involved in some of the molecules and genes and found gene expression patterns of expression and regulation of signaling pathways.Conclusion:LncRNA RP in the peripheral blood, the presence of clinically significant characteristic expression profiles associated with the pathogenesis.