Study On The Effect Of The Effective Components Of Lcssy On The Angiogenesis Of Ovarian Cancer And The Regulation Mechanism Of JAK2/STAT3 Signaling Pathway

Objective:To screen the differential expreesion genes in Balb/C nude mice subcutaneous transplantation ovarian tumor treated with lichong Sheng-sui Yin(LCSSY) effective component by gene chip,expore the mechanism of treating ovarian cancer;Based on the results of gene chip,detect the mechan-ism and validation of LCSSY effcetive component on ovarian cancer angiogenesis.Method:To establish the model of ovarian cancer in Balb/C nude mice, and then randomly divided into model group, bevacizumab group, low-dose group of LCSSY, LCSSY dose group, high dose of LCSSY group.Measure the tumor volume during the course of treatment and weigh the tumor in the end of treatment. By gene chip technology screening the difference of gene expression in dose group and model group, to explore the mechanism; And detect the tumor tissue angiogenesis by immunohistochemical method;then detecte JAK2 and STAT3 and VEGF mRNA expression with qPCR in tumor tissue; Western blotting detect JAK2 and STAT3 and VEGF protein expression in tumor tissue. To verify the effection and mechanism of the treatment of Chinese medcine on the angiogenesis of ovarian cancer.Results:Lcssy effective component three dose group and bevacizumab group have significant effective in inhibit ovarian cancer tumor growth, compared with Lcssy effective component three dose group, the effection is better in bevacizumab,and compared with the model group (P<0.01); The analysis of gene microarray show that there are eleven differential expreesion genes associated with ovarian cancer angiogenesis; immunohistochemistry results show that bevacizumab derugulate the tumor tissue VEGF, CD34 expression, reduce MVD, compared with the model group had significant difference (P<0.01); lcssy effective components three dose group can significantly derugulate the VEGF, CD34 expression, and compared with the model group had significant difference (P<0.01). the result of qPCR show that LCSSY different dose groups can reduce the expression of JAK2, STAT3 and VEGFmRNA in tumor tissue (P<0.01). The result of Western blot show that LCSSY reduce the amount of JAK2, STAT3, VEGF protein expression in tumor tissue and show a dose effect correlation (P<0.01).Conclusion:LCSSY effective component can significantly inhibit the growth of ovarian cancer.LCSSY can inhibit VEGF,CD34 expression in ovarian cancer to inhibite tumor angiogenesis; LCSSY can regulat the expression of JAK2 and STAT3 mRNA and protein in ovarian cancer; the fuction of refrain tumor angiogenesis of LCSSY is associate with JAK2/ STAT3 signal pathway.