Study Of Cerebral Cortex Development Affected By Congenital Kidney Deficiency Based On Reelin Protein Molecular Mechanism

Objective The method of "fear of injury pregnant rat" was used and typical kidney essence deficiency models were created in our experiments.The influences of Reelin protein on congenital kidney deficiency development mechanism in new born rats were studied.Mthod ①40 three-month old female and 20 male SD(Sprague Dawley) rats were involved in this experiment.Sex ratio in cage was 2:1. Female rats were randomly divided into normal group,model group and kidney recovery group after conception.Each group involved 10 individuals.Daily composite stress stimulation were given to model group and kidney recovery group since the first day of pregnancy.Kidney group since building the first day, a day to the traditional Chinese medicine(TCM) zuo gui wan thickener 2 ml / 100 g weight to fill the stomach, the normal group and model group and saline lavage, according to the weight of female die during the time for the entire pregnancy and childbirth. Female mice after childbirth, calculate each female each tire production number(wang rat/total number of pregnancy and childbirth females only) to evaluate the fertility of the females.Weighing between groups that females give birth rat weight and measurement of primary rat body length. ② Wang rat was born on the day of the random take 1-2 per litter boy rat beheaded in whole brain, according to whole brain wet weight, calculating the average groups of young rats all wet weight of the brain and brain weight index(brain weight index = whole brain wet weight mg/g) rat weight, then the whole brain fixed, take contain cortex of brain slices, light microscope and electron microscope. ③ Female mice after childbirth, in rat blood, was born the day break with chemiluminescence detection of serum FT3, FT4, TSH levels. ④ Using immunohistochemical method to detect a fear of injury pregnant rat rat brain on its seed Reelin, VLDLR and P- DAB1 expression effect. ⑤ Fear of injury by rt-pcr method pregnant rat rat cerebral cortex on wang Reelin, mRNA expression of VLDLR and ApoER2 influence.Results ① Model group of pregnant rats each tire production number is lower than normal group and kidney group significantly(P < 0.01).Model group young rat was born on the day of the weight and length compared with normal group and the kidney, with significant difference(P < 0.05). ② Whole brain wet weight of the rat model group boy is lower than the normal group and kidney group, model group compared with normal group had significant difference(P < 0.05) and normal group compared with the whole brain wet weight of the rat kidney group boy has no obvious change;Wang rat brain tissue including hippocampus morphology change: model group young rat brain tissue including angiohyalinosis(see the decrease in the number of nerve cells in the hippocampus, interstitial edema, neuron dendritic branches to reduce.Under the electron microscope, most of the nerve cell organelles structure have abnormal, part of the nucleus pycnosis.The seed morphology of the rat kidney group and normal group compared with no obvious change. ③ Normal group, model group and kidney content comparison of three groups of serum FT3, TSH, as a result there was no significant difference;Model group compared with normal group and the kidney, the serum FT4 content decreased significantly(P < 0.05). ④i n the normal group of young rat cerebral cortex cells Reelin, VLDLR and P- the strongest DAB1 protein expression;Kidney group of young rat cerebral cortex cell Reelin, VLDLR and P- DAB1 protein expression reduced;Model group Reelin, VLDLR in rat cerebral cortex cells and P- DAB1 protein expression is weakest;Compared with normal control group, and group and model group wang rat cerebral cortex Reelin, VLDLR and P- DAB1 average grey value significantly decreased(P < 0.01);Compared with model group, kidney group of young rat cerebral cortex Reelin, VLDLR and P- the average grey value of DAB1 significantly increased(P < 0.01). ⑤ Kidney group and model group rat brain cortex Reelin, VLDLR and ApoER2 mRNA expression were lower than normal group(P < 0.01), model group rat cerebral cortex Reelin, VLDLR and ApoER2 mRNA expression below kidney group(P < 0.01).Conclusion This topic first puts forward the "Reelin protein for congenital kidney empty wang rat cerebral cortex effects" point of view, and from the kidney as well as the influence on brain structure and function of the Angle of the relationship between Reelin and brain development carried on the thorough theory discussed in this paper.This study adopts the method of "fear of injury pregnant rat" successfully reproduce the kidney essence deficiency model, found that the performance of the model group wang mice with congenital kidney empty, the development of cerebral cortex obvious obstacles, such as cerebral cortex, including the hippocampus brain cell nucleus pycnosis, condensed chromatin and nuclear weeks edema, markedly swollen mitochondria, part of the peak disappeared, golgi complex hypertrophy.Traditional Chinese medicine(TCM) left to pill can effectively block the congenital kidney affect brain development.Model group Reelin, VLDLR in rat cerebral cortex cells and P- DAB1 protein expression, Reelin, VLDLR and cut ApoER2 mRNA expression, serum TH content dropped significantly.Therefore, we hypothesized that reelin signaling pathways and a significant reduction in the content of TH is an important link in congenital kidney empty seed rat brain developmental disorders.