| Peripheral nerve injury is essentially of axonal injury， Neurotrophic factorplummeted after peripheral nerve injury axonal retrograde transport provides,leading to neuronal death, weak regeneration.Research shows that the neurotrophicsubstance, synthesis of target tissue regeneration and repair for injured nerve.Nervegrowth factor (nerve growth factors, NGFs) is a neurotrophic factor mainly, onneuronal survival, growth,differentiation, regeneration and maintenance of functionplays a very important role in nutrition and regulation.But NGF can not exist defectsthrough the blood brain barrier, toxic and side effect of systemic administrationoflarge, biological activity in the aqueous solution of short half-life, based on this weuse polyethylene/poly (ethylester of-L-gamma glutamic acid)(PEG-PELG)hydrogel supported NGF, prepared with slow release ability of the new dosage formNGF.In vitro tests confirmed based combined application of NGF and VPA havesynergistic effect to promote the differentiation of PC12cells, the animal model ofWistar after sciatic nerve injury in rats to create silicon tube nerve regenerationchamber, the new forms of NGF (injected into the neural regeneration chamber) andVPA (oral) are used in combination, the observation after injury of the nerve axonsregeneration of the quantity, quality and electrophysiological recovery of functionchange.The experimental results show:1, B, C, D, E, F, G groups of electromyography in the D42point in time recoveryperiod of sciatic nerve showed,through the nerve regeneration chamber MUAPwaveform generated by the irregular distal, discrete, extend the time limit, thewaveform of each section is not exactly the same, after nerve regeneration chamberwere significantly AMP attenuation,At the same time after CV had the nerveregeneration chamber were significantly slower, compared with A, were statisticallysignificant (P<0.05); wherein there are differences between D, E, compared F, G fourgroups, but no statistical significance (P>0.05), B, C between the two groups had littledifference had no statistical significance(P>0.05), D, E G, F, B, C four group and two group compared with the decay rate of AMP CV increased significantly smaller, therewas statistical difference (P<0.05).2, the average space groups observed myelin nerveregeneration, axon from the total area and relative recoveryrate results show thatgroups of nerve regeneration through the anastomosis:A group of axons myelin isthicker, more in number, average diameter is big, the other groups were observedunder a light microscope to see increase mesenchyma, axon myelin small, scattereddistribution, calculated the total area was significantly higher than the blank groupaxon axon area reduced, the recovery rate between groupsalso have differences, therewas a significant difference in comparison with the A group (P<0.05) between thegroups were compared and analyzed, including E, F, G three groups of axons andaxon area total area recovery rate between groups had no statistical difference(P>0.05), the recovery rate of E, F, G three groups of axons andaxon area total arearespectively were higher than those of B, C, D three groups with statisticaldifference(P<0.05); recovery rate of D group and the total area of axon axon area ishigher than that of B and C with statistically significant differences between the twogroups (P<0.05); B, C two groups of axons and axon area total area recovery ratecompared no significant difference between groups (P>0.05).Conclusions:Silicone tube neural regeneration chamber using new forms of NGF canpromote nerve regeneration,and can exhibit better than high dose effect of low dose.Low dose of the new forms of NGF combined with VPAand the effect of combinedhigh dose VPA and high dose effect is similar, with synergistic effect. Nerveregeneration chamber model silicone tube can effectively promote nerve regenerationmedicine effectdetermination. |
