Viscosity Fluorescence Probes Of Targeting Cell Lysosome Or Chromatin

Organic fluorescent dyes played a vital role in biomedical field, such as biology chemistry, ion detection, fluorescence imaging microscopy, cell microenvironment, and received widespread attention. However, most fluorescent dyes don’t have a targeting group, the dyes can’t meet testing in the cell specific microenvironment and key physiological processes. In this paper, the structure of BODIPY dyes were transformed and synthesized microenvironment viscosity probe for lysosome and chromatin, which was used to research the important physiological process of cell.(1) BODIPY was designed and synthesized lysosome viscosity probe (Lyso-V) with a targeted group (Morpholine). Morpholine’ alkaline tended to make Lyso-V into lysosome. The rotor rotation caused the changes in Lyso-V’s fluorescence lifetime to quantify lysosome viscosity. Lyso-V was protonated in the lysosome, which was used for real-time tracking and monitoring the lysosome viscosity changes. The intrinsic fluorescence of Lyso-V is weak, based on photo-induced electron transfer (PET) in a non-acidic environment. Even if a small amount Lyso-V was leaked, the background fluorescence was negligible. The viscosity rotor could rotate freely. Lyso-V has low cytotoxicity.(2) BODIPY was synthesized chromatin viscosity probe (Chroma-V) with a targeted group (Hoechst). Chroma-V has been obtained by Click reaction between Hoechst and BODIPY. Hoechst groups tend to bundle the minor groove of double-stranded DNA with a preference for sequences rich in adenine and thymine, and make Chroma-V for staining chromatin in nucleus. In the normal, apoptosis and aging cells, chromatin’s viscosity was detected with fluorescence lifetime imaging. Chroma-V has a better cell permeability and low cytotoxicity.(3) BODIPY dyes have small stokes shift (10nm) is disadvantaged, which will cause quenching in high concentration. In this paper, three BODIPY dyes (3a-3c) were designed and synthesized with large stokes shift and high fluorescence quantum yield.3a-3c showed large stokes shift (50nm), high fluorescence quantum yield (0.8), large molar extinction coefficient (65000), excellent light stability, and the influence of solvent polarity could be ignored.