Study Of Biological Mechanisms Of Yiqihuoxueqingjin Prescription On Pulmonary Fiborsis Of Rat Induced By Bleomycin

Objective:Based on TGF-β/Smad signaling system, to probe the possible biological mechanisms of Yiqihuoxueqingjin prescription on pulmonary fibrosis of rat induced by Bleomycin.Method:The96SD male rats which weighed200±20gramme were randomly divided into four groups:Control group(K), Model group(BLM), Positive control group(P)and Drug group(Y). Each group of rats were once injected with bleomycin through the trachea what the dosage was5mg/kg except for the control group which were injected with equal dosage of saline.The K and BLM groups of rats were fed with saline(10g/kgd), the P group of rats with prednisone(4.2mg/kgd) and the Y group of rats with Yiqihuoxueqingjin prescription(9.45g/kgd) by gastric gavage each day. The animals were respectively put to death in the7th day,14th day,28th day. We collected serum in order to detect PCIIINP, C IV, HA and LN. At the same time, we dissected the lung for HE and Masson staining, analyzing C I, TGF-β RI, TGF-0RII, Smad3, Smad7by immunohistochemical method and detecting the gene expression of TGF-β1mRNA, Smad3mRNA, Smad7mRNA by RT-PCR.Results:The relvent indexes between the K group and BLM groups were statistically different(P<0.05).The Y group can improve the general condition of the animals compared to the BLM groups, and it helped to gain in weight normally, to prevent the wet weight of the lung from increasing excessively, and improve the lung coefficient. The results of HE staining:In the28th day the degree of inflammatory cell infiltration of the Y group was significantly better than the BLM group, and there were no fibrous scar. The results of Masson staining were similar with that of HE staining. The detected results of PCIIINP, C IV, HA, LN by radioimmanoassay:Each detected indexes of the Y group were statistically different(P<0.05) from that of the BLM group in the14th and28th day. MIOD of C I, TGF-βRI, TGF-βRII, Smad3, Smad7by immunohistochemical method:Each indexes of the Y group were statistically different(P<0.05) from that of the BLM group at each time point in addition to that of TGF-βR I in the7th and14th day and that of Smad7in the7th day. The outcome of TGF-β1mRNA, Smad3mRNA. Smad7mRNA by means of real-time quantitative polymerase chain reaction:There was statistically significant difference between the Y and BLM group(P<0.05) at each time point in addition to that of Smad3mRNA and Smad7mRNA in7th day.Conclusion:Yiqihuoxueqingjin prescription can inhibit pulmonary fibrosis from progressing by means of influencing on related links of the TGF-β/Smad signaling system.