Based On Mass Spectrometry Imaging, Establish The Methods To Analyse The Differences Of Lipidomics Between Nasopharyngeal Carcinoma Tissues And Normal Tissues

ObjectiveBased on mass spectrometry imaging, initially establish the methods to analyse the differences of lipidomics between nasopharyngeal carcinoma tissues and normal tissues. We hope to discover some biomarkers that can help diagnosis, predict prognosis, build new metabolic grouping methods and guide treatments. Then we can find screen nasopharyngeal carcinoma more sensitively and treat them more effectively.Methods24 nasopharyngeal carcinoma tissue samples and 10 chronic nasal mucus inflammation tissue samples were collected. After being paired, every single sample goes through scan by LC-MS. We observe the differences between tumor tissue area and the area just beside the tumor tissue on the same sample. At the same time, we also show interest in differences between tumor tissue samples and normal tissue samples.ResultsThrough MSI, we can know many kinds of m/z values from metabolites and the peaks of these m/z values in tumor tissue samples or normal tissue samples. When we chose certain m/z value, we discover that the mass spectrometry images match the HE images basically. On the same scale, we can compare the relative metabolite contents between tumor tissue samples with normal tissue samples. In the same sample, We observe the relative metabolite contents between tumor tissue area and the area just beside the tumor tissue.ConclusionThrough MSI, we can know many kinds of m/z values from metabolites and the peaks of these m/z values in certain sample. When we chose certain m/z value, we get the size and shape of the sample according to MSI. On the same scale, we can compare the relative metabolite contents between tumor tissue samples with normal tissue samples. In the same sample, We can compare the relative metabolite contents between tumor tissue area and the area just beside the tumor tissue. We have initially established the methods to analyse the differences of lipidomics between nasopharyngeal carcinoma tissues and normal tissues based on mass spectrometry imaging. In the future, we will combine multivariate analysis with nasopharyngeal genetic maps, metabolic mechanics to explore the metabolic differences between tumor tissues and normal tissue systematically.