| Objective:the influence of different doses of Munziqs on myocardial ischemia reperfusion injury (MIRI) rat with abnormal savda and its mechanism were studied. Methods:On the basis of previous studies, we constracted myocardial ischemia reperfusion injury (MIRI) rat with abnormal savda and performed the model validation study. On the basis of MIRI rat with abnormal savda model construction, to explore the influence of different doses of Munziqs on myocardial ischemia reperfusion injury (MIRI) rat with abnormal savda and its mechanism, we selected84male Wistar rats were divided into7groups with12rats per group, including mode ischemia reperfusion group, large dosed Munziqs group, medium dosed Munziqs group, small dosed Munziqs group, normal ischemia reperfusion group, mode false surgical group and normal false surgical group. The heart physiological situation and myocardial morphology change after injury of each group during MIRI were monitored; To further reveal the protection mechanism of abnormal savda Munziq on ischemia-reperfusion injury in myocardial, we select96rats to divide them into eight groups:ischemia-reperfusion model group, high dose maturation agent group, middle dose group mature agent, small doses of maturation agent group, normal ischemia-reperfusion group, model sham group and atorvastatin intervention group (positive control group). Each group contained12rats. The expression of myocardial tissue HSP70, CGRP were detected by Western blot method, the serum MAD, SOD, interleukin-6and interleukin-8levels were detected with ELISA kit. Results:(1) According to the relevant literatures and on the basis of previous work, we successfully constructed the abnormal savda ischemia-reperfusion injury model and validated that this rat model can be carried through subsequent research.(2) We found in Munziq intervention group, the serum creatine kinase and troponin levels were significantly lower than those in the model group, the middle dose group showed the lowest levels. The HE staining of myocardial tissue changes showed that the myocardial edema and muscle fiber proliferation levels were significantly higher in the Munziq intervention group than those in the model group. The middle dose group showed the lightest cardiac tissue damage.(3) compared with normal ischemia reperfusion group, the ventricular premature contractions (VPC) incurring time in groups with Munziqs intervened was significantly delayed, and the accumulative time was reduced. Among of them, the large dosed Munziqs group was most significant. There was no delayed VPC incurring time in small dosed group, but with significantly reduced accumulative time (P<0.01); there was no significant difference of ventricular tachycardia (VT) incurring time between each group.(4) compared with normal sham group, HSP70expression in ischemia reperfusion groups were significantly increased (P<0.01); compared with ischemia reperfusion groups, HSP70expression in groups with Munziqs intervened were significantly enhanced (P<0.01). Compared to the reperfusion group, the SOD levele significant increased and the MDA, interleukin-8and interleukin-6levels significantly decreased (P<0.01) in the Munziqs intervention group;(5) Compared to low dose Munziqs group, there were not significantly differences in HSP70, CGRP, MDA, interleukin-8and interleukin-6levels in atorvastatin intervention group; However, there was significant defference in HSP70expression between high dose Munizq group and atorvastatin intervention group (P<0.05), although the CGRP expression and the serum SOD, MDA and interleukin-8and interleukin-6levels showed no differences between the large doses of Muniqs intervention group and atorvastatin intervention group. Conclusion:1) We successfully constructed the Abnormal Savda ischemia-reperfusion model whih can be used for subsequent studies;2) Munziqs intervening can improve the heart physiological function of mode rats with MIRI, and increase the expression of protective protein HSP70, While obviously reduce myocardial ischemia-reperfusion myocardial inflammation.And it has significant dose-dependent.3) Moderate to high doses of abnormal savda Munziq may be more effective on abnormal savda ischemia-reperfusion injury in rats than atorvastatin, but still need further studies to confirm the conclusion. |
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