Effect Of Adenovirus Infection On The Inflammation And Apoptosis Of CSE Induced Emphysema Mice

Objective: To investigate the effect of adenovirus infection on the CSE(cigarette smoke extract) induced emphysema mice from the aspects of.pulmonary function, pathology, inflammation, protease, apoptosis and proliferation.Methods:BALB/c mice were injected with PBS/CSE at day1,11and22, and were received PBS/adenovirus intratracheally at day15. Mice were sacrificed at day28. Fluorescence of red fluorecent protein, pulmonary function, pathology of lung tissue by using mean linear intercept(MLI), destructive index(DI) and mean alveolar septal thickness(MAST), score of inflammation, expression of MMP-2and MMP-9, apoptosis and proliferation of lung tissue were measured.Results:(1) There was red fluorescence observed in CSE+Adenovirus group, while no red fluorescence in PBS and CSE group.(2) Compared with PBS group, airway resistance(RAW) was higher in CSE group and CSE+Adenovirus group(p<0.05), and lung dynamic compliance(Cdyn) was lower in CSE group and CSE+Adenovirus group(p<0.05). There were no significant differences in RAW and Cdyn between CSE group and CSE+Adenovirus group(p>0.05).(3) Compared with PBS group, MLI and DI were higher in CSE group and CSE+Adenovirus group(p<0.05), and MAST was lower in CSE group and CSE+Adenovirus group(p<0.05). There were no significant differences in MLI, DI and Cdyn between CSE group and CSE+Adenovirus group(p>0.05).(4) There were lots of inflammatory cells around small airway and microvessels in CSE+Adenovirus group. Compared with PBS group and CSE group, the scores of inflammation in small airway and microvessels were higher in CSE+Adenovirus group(p<0.05). There were no significant differences in the scores of inflammation in small airway and microvessels between PBS group and CSE group (p>0.05).(5) Compared with PBS group, the expression of MMP-2and MMP-9increased in CSE group and CSE+Adenovirus group(p<0.05). Compared with CSE group, the expression of MMP-2and MMP-9increased in CSE+Adenovirus group(p<0.05)(6) Compared with PBS group, the apoptosis index(DI) was higher in CSE group and CSE+Adenovirus group(p<0.05). Compared with CSE group, DI was higher in CSE+Adenovirus group(p<0.05).(7) Compared with PBS group, the proliferation index(PI) was higher in CSE group and CSE+Adenovirus group(p<0.05). There were no significant differences in PI between CSE group and CSE+Adenovirus group (p>0.05).Conclusions:CSE intraperitoneal injection can induce emphysema mice successfully. Adenovirus infection can promote the inflammation, increase protease expression and enhance apoptosis level.