Experimental Study Of Peripheral Nerve DEF ECTS Repaired By Collagen Nerve Sheath

Background Currently, peripheral nerve injury caused by defects inthe treatment of major surgical therapy. Which is greater than5mm nervedefect is the gold standard for the treatment of nerve autograft, but thistreatment is accompanied by a variety of clinical complications; Nowmany researchers have done a lot in-depth research, hoping to find an idealnerve induced bridging material. Objective comparative study of collagen-induced rabbit nerve sheath surrounding nerve Regeneration effect, tofind a short segment (<20mm) ideal for bridging nerve defects thereof.Methods72different species of New Zealand white rabbits were randomlydivided into three groups, cut off the right side of the sciatic nerve, thenerve defect model is made of20mm; using collagen nerve sheath as theexperimental group A bridge to bridge silicone tube as the group B, nerveautograft was used as control group C, n=24rabbits. At2weeks aftersurgery, compared to the situation in each group immunohistochemistry toevaluate the immunogenicity of each group, nine weeks after surgery,biopsy and histological evaluation of image analysis, comparing the resultsof each group of nerve regeneration and repair of the defect, after12weeks by general observation, comparison measurements tibialis anterior muscle wet weight and evaluate their recovery rate, to detect the nerveelectrophysiological conduction velocity, and the whole incubation period,after nine weeks, histological sectioning and image analysis andevaluation, comparing the groups of nerve regeneration defect repairresults. Results: After two weeks, A and C group close transplantedsegment Wallerian degeneration lighter, obscurely, a few fibroblasts, SClot, arranged regularly, did not see the LC cell infiltration, group B graftsWallerian degeneration obvious, fibroblasts more, SC more aligned thanthe rule, visible LC infiltration. A group at12weeks after the nerve hasregenerated into the distal catheter through the nerves and the regenerationof nerve fibers grow thick, while group C nerve fibers regenerated throughthick long catheter into the distal nerve; A, group C regenerated nerve lookcloser, B group at12weeks after nerve fiber growth by bridging thecatheter, smaller nerve fibers, in the regeneration of nerve conductionvelocity: A group A and group C was significantly better than group B (P<0.05); in A, B, C three groups muscle atrophy, the group B and therecovery rate of contraction of the most serious difference. Group A,group C had no significant degree of muscle atrophy and recovery ratequite. Image Analysis nerve fiber counts up to a maximum group C, Agroup followed with B differences were statistically significant compared(P <0.05); A, the number of nerve fibers in group C, the degree of maturityto be significantly better than group B. In this study, after3months, the electron microscope: A and group C regenerated nerve myelin thickstructural rules, evenly distributed, high maturity. Group B regeneratednerve myelin disorder thin structure, maturity is not high. A regenerationof nerve regeneration and group C was significantly better than the groupof neural structures B. Conclusion1collagen nerve sheath has goodbiocompatibility, absorbable, weak antigenicity.2collagen nerveregeneration nerve sheath bridging group, the myelin sheath thickness,maturity, significantly better than the three-dimensional structure group B,the effect of autologous nerve graft closer.3three bridging nerve defectswithin the material contrast20mm collagen nerve sheath in promotingsciatic nerve regeneration, nerve regeneration arranged laws, improvenerve myelination, accelerate reconstruction and regeneration of nervefunction were superior to silicone tube group, the effect of autologousnerve graft closer.