| Objectives:Taxol is a kind of anti-tumor drug that are widely used in a variety of malignant tumors,but the emergence of taxol resistance badly damaged its chemotherapy effect. The study of paclitaxel resistant mechanism has become a research hotspot, but none of them clarify the mechanism quite clearly. In recent years, the found of a new gene named TXR1(Taxol alcohol resistance gene1)provides a new idea for cancer research, but its function and the intracellular signal pathways are still not clear, the purpose of this study was to Explore the role of TXR1playing in the process of paclitaxel-resistance in nasopharyngeal carcinoma cellsMethods:1.After being transfected by TXR1-22,the expression of TXR1gene was detected by RT-PCR and Western Blot in one strain of paclitaxel-resistance cells named CNE-1/Taxol.2.The sensitivity to paclitaxel of the cell above was detected by MTT test.3.The growth of NP69and CNE-1after being transfected by TXR1-22were assessed by MTT test.4.Four genes named TXR1,TSP1,CD47,DUSP1were selected by bioinformatics and their relationship among them were detected by RT-PCR.Results:1.It showed that TXR1expression was obviously inhibited in CNE-1/Taxol by RT-PCR and Western blot.2.The sensitivity to paclitaxel was partially increased.3.The growth of NP69and CNE-1were not significantly restrained.4.RT-PCR showed that mRNA of all of the three genes above were significantly upregulated by silencing TXR1.Conclusions:1.It was further proved that TXR1had a close relationship with taxol resistance.2.It indicated that the FOLR1gene might be one of the important target molecules in the reversion of the paclitaxel-resistance in nasopharyngeal carcinoma cell.3.The thress genes above may played important roles in TXR1induced taxol resistance... |
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