Study On Transcriptional Level Of LncRNA In Paravertebral Muscle Of Adolescent Idiopathic Scoliosis

Background:Idiopathic scoliosis is a spinal deformity, accounting for80%of scoliosis patients, which has a serious impact on life quality. Based on the age of onset, idiopathic scoliosis can be subdivided into three groups—infantile idiopathic scoliosis, aged0-3years; juvenile idiopathic scoliosis, aged3-9years; and adolescent idiopathic scoliosis, aged10-16years. About80patients of idiopathic scoliosis are adolescent idiopathic scoliosis. The aetiopathogenesis of AIS remains unknown.There are already many hypotheses, which can not explain the mechanism of adolescent idiopathic scoliosis well.Studies indicate that AIS has the characteristic of inheritance. Many researches in genomics and proteomic has been done, but there is not a good explanation to the mechanism of adolescent idiopathic scoliosis. Long noncoding RNAs （lncRNAs） are defined as transcripts longer than200nucleotides without function of coding protein. Recent researches find that lncRNAs act as regulator of gene expression by diverse ways,which play important roles in the onset or development of disease. In this study, we focus on lncRNAs, and screen lncRNAs of interest with the technique of gene chip. Through detect the transcription level of lncRNAs in paravertebral muscle of AIS with quantitative PCR, we wish more information on aetiopathogenesis of AIS can be discovered.Objectives:1. To select the lncRNAs which are specific in AIS,as well have effect on BHiiscutosfeeletal-systenvwith-the technique of gene chip from peripheral blood;2. To analyse the diffence of the transcription level in case-contol and different group of AIS through detecting transcription level of the lncRNAs in paravertebral muscle with the technique of qPCR. Methods:1. Peripheral blood was collected from4patients with AIS and4age-and gender-matched normal children and then total RNA was isolated and tested with Agilent human lncRNA+mRNA Array V3.0. Base on the latest KEGG （Kyoto Encyclopedia of Genes and Genomes） database, with pathway analysis and GO analysis,we selcet the lncRNAs which are specific in AIS,as well have effect on musculoskeletal system.2. paravertebral muscle was collected from21patients with AIS and4patients with Intervertebral disc protrusion. With the techqiue of qPCR, we detect the transcription level of the lncRNAs in paravertebral muscle.Results:1.5AIS specific lncRNAs which may have effect on musculoskeletal system are selectd, they are ENST00000602322.1、HIT000067310、 ENST00000440778.1、ENST00000414894.1、TCONS₀0028768;2. These lncRNAs all have low transcript level in AIS patients（P<0.05）;3. There is no significant difference when comparing concave with convex in AIS patients（P<0.05）.4. When patients with AIS were grouped according to clinical features, including age, menarche, everity of scoliosis measured by Cobb angle, Risser grade, transcript level differences were found. There is low transcription level of ENST00000440778.1and TCONS₀0028768in age of menarche<12（P<0.05）.Low transcript level of ENST00000602322.1is found in group of Risser grade>I （P<0.05）. And we can find patients in group of Cobb angle<47have low transcript level of HIT000067310（P<0.05）.Conclusions:1. In this study, we discover the lncRNAs of ENST00000602322.1、 HIT000067310、ENST00000440778.1、ENST00000414894.1、TCONS00028768 have low transcription level in AIS patients.2. The transcription level of hese lncRNAs have no significant difference in convex and concave of paravertebral muscle.3. This lncRNAs have different transcription leve in different clinical group of AIS patients.