To Therapy In-Stent Restenosis After PCI By Targeted Inhibiting The Cell Cycle Of VSMC On A Rat Model

Objective:The present study was to establish the balloon injury model on rats’carotid artery, to suppress neointimal hyperplasia specifically by targeted inhibiting the cell cycle of vascular smooth muscle cell, and to achieve the goal of suppressing restenosis after PCI without affecting the repair of endothelium.MethodAfter injury of the left carotid artery by balloon, we infected the vascular with Lenti-SM22a-P27-EGFP, Lenti-SM22a-EGFP or PBS, observed the neointimal hyperplasia in the three groups and sham group at the time points of7,14and28days after the operation, observed the expression of P27, PCNA and vWF at the time points of7,14and28days after the operation of all four groups.Results:The neointimal hyperplasia significantly decreased on14or28days After infecting with Lenti-SM22a-P27-EGFP compared with control group (P<0.05or P<0.01), meanwhile, the repair of endothelium did not differ with other groups.Conclusion:The pathological process of restenosis after PCI could be mimicked by the model of balloon injury on rats’carotid artery, Lenti-SM22a-P27-EGFP could specifically inhibit the abnormal proliferation of VSMC after PCI and did not influence the endothelium, so as to suppress restenosis effectively without affecting vascular endothelialization.