MBD2as A Potential Target For Treatment Of Hematopoietic Malignancies

Background and objectives; An experimental study was performed to analyze the expression of MBD2protein in hematopoietic malignancies, the correlation status with MBD2mRNA, also detecting the role of MBD2protein in hematopoietic malignancies and as a target for therapy.Method: K562, JURKAT and THP-1and others cell lines were used as a model for our study, MBD2protein expression was analyzed by Western Blot. MBD2mRNA was analyzed by Real Time-PCR, lentivirus shRNA-MBD2was used to knock down MBD2protein. Apoptosis, cell cycle, colony forming Cell assay were done and analyzed by flow cytometery, treatment sensitivity of MBD2knocked down cells also was performed.RESULTS: There were expression and correlation in MBD2Protein and mRNA in hematopoietic cell lines, after knocking down MBD2protein the apoptosis of cells was increased for K562and JURKAT, while did not affect on THP-1, the cell cycle demonstrated no significant change comparing to negative control cells, colony forming cell assay done for THP-1demonstrated significant reduction in proliferation. Treated MBD2knocked down THP-1cells with DNR demonstrated significant increase in Apoptosis, while cell cycle analysis showed no significant change in S-phase, there were slightly increase and decrease between GO and G1phase.CONCLUSION:hematopoietic malignant cells express MBD2protein, this protein has significant role in tumorgenesis, can be used as a target therapy for killing and decreasing proliferation of malignant hematopoietic cells.