Comparison Of Side Population Cell Sorting Method And Preplate Technique In Extraction Of Skeletal Muscle-derived Stem Cell

Objective To verify the existence of side population cells in skeletal muscle; To verify side population cell sorting method can be used to purify muscle-derived stem cells; To compare the differences between the two methods of side population cell sorting method and preplate technique。Methods Get skeletal muscles from the hind leg of6weeks SD rats,and skeletal muscle stem cells were separated and extracted, then side population cells method and preplate method were used tu purify the skeletal muscle stem cells, and finally the test the cells got from the two different methods by immunohistochemistry, Western blot and PCR analysis, and compared the differences between the two groups.Results Side population cells are found in skeletal muscle cells。 Desmin, Sca-1, CD34the three muscle-derived stem cell markers were positive in the cells got by the side population cells method,and the expression intensity difference was not significant from the cells got by the preplate method.Conclusion The presence of side population cells in skeletal muscle; In the side population cells there are a lot of muscle-derived stem cells, and side population cells method can be used to extract muscle-derived stem cells; Side population cell sorting method is more provinces, and convenient than preplate technique。...