| Much emphasis has been put on the study of early implant-bone interface, fewstudies have focused on peri-implant soft tissue interface. In spite of the high successrate of dental implants in the modern era, lots of implant failure cases have beenreported. Clinical data shows that, because of the problems happened in gingivalhealing program,17%patients will appear infection. The poor healing ability betweendental implant and soft tissue is an important factor to the peri-implantitis, therefore,the treatment of dental implant surface is one of the most important factors whichdecides the success or failure of dental implants.It has been reported that, the reactions to different gingival soft tissue and bacteriadiffers from one dental implant surface treatment to another. With goodosseointegration properties, micro-arc oxidation is now gradually become the key point in basic research and clinical trials, but interface between the implant surface treatedby micro-arc oxidation and gingival soft tissues have been seldomly reported.Our study focused on the interface between micro-arc oxidation treated surface oftitanium implant and gingival soft tissue. The influences of micro-arc oxidation surfacetreatment on the biological behavior of human gingival epithelium and commonpathogen Streptococcus mutans have been studied, hoping to provide more data for theclinical application of micro arc oxidation treatment on pure titanium implants.Methods:Firstly, prepare the characteristic of micropore structure of implant test specimenwith commercial pure titanium, and epithelial cells were cultured from normal humangingival soft tissue cut through the guide of orthodontic adorable harvested, enoughhigh purity human gingival epithelial cells (hGEC) can be obtained by then. On thisbasis, the changes of the adhesion and proliferation of human gingival epithelial cells,and of the expression of epidermal growth factor and related genes were observed, toevaluate the influence of microarc oxidation surface treatment technology for fabricinterface of titanium implant and gingival soft group, and further on Streptococcusmutans in materials surface adhesion and proliferation were studied.Results：1Scanning electron microscope observation on the cell morphology showed byhuman gingival epithelial cells in cell adhesion in two groups of samples surface aregood, no obvious difference was found between the two groups in the number ofadherent cells within the same hemifield.2MTT method was taken to test the preliferation level of human gingivalepithelial cells on different treatet surfaces, including and polished pure titaniumsurface. The number of human gingival epithelial cells inoculated0.5hours,1hours,2hours on the surface of micro-arc oxidation treated surface was higher than that of pure titanium surface polishing treatment (P), but no statistically significant was found(P>0.05).3Detection of proliferaton rate of human gingival epithelial cells was taken byMTT method. The results showed that the cells cultured on micro-arc oxidation treatedpure titanium surface (MAO group) in the inoculated cells after the first and secondday proliferated rapidly, and the number of cells was significantly higher than that ofpure titanium surface polishing treatment (group P)(P <0.05). However, cellproliferation rate on two groups of specimens surface has slowed down since the fifthday, and there was no significant difference between the number of cells on the surfaceof the two groups (P>0.05).4ELISA was used to analysis the influence of different surface treatment on theexpresson of epidermal growth factor, the data showed that no statistically significantdifference was found between two groups after7days inoculation of human gingivalepithelial cell(P>0.05).5The result of real time PCR showed that, Itg β1, E-cad and PCNA were allexpressed on the surefaces two groups at the first day; no significantly defference wasfound between the expression of Itgβ1and PCNA at1,3,5days (P>0.05); E-cad onthe first day of micro-arc oxidation treatment surface expression level is higher thanthat of polishing group (P <0.05), but no significant difference of the expression levelswas found between the two surface treatment at3,5days (P>0.05); expression levelof EGF is very low at the first day in two groups of test piece surface, on the third dayand the fifth day, the expression level increased significantly as the culture timeextended, but no significant differences were found in different surface treatmentbetween the two groups (P>0.05).6The on the Streptococcus mutans inoculated on different titanium surfacedisplayed tha: I, few adhesion and proliferation of Streptococcus mutans were found innumbers of polished titanium surface, small groups clusters scattered along the tracespolishing surface texture was observed, with the time of culture prolonged, the number of Streptococcus mutans on the specimen surface gradually increased. II On puretitanium surface treated by micro-arc oxidation, Streptococcus mutans growed in theholes formed by microarc oxidation surface treatment as the center to the surroundingdiffuse distribution, number of the adhesion and proliferation were reletived larger,and with the prolongation of culture time, the number of bacteria on the surface wasalso gradually increasing. III In polished titanium group, the number of Streptococcusmutans deffered between4hours and24hours cultured group (P <0.05), but in4hours of training group and training group for8hours,8hours between training groupand the culture of Streptococcus mutans in number between the hours of24groupswere not significantly different (P>0.05). IV For micro-arc oxidation treated group,the number of Streptococcus mutans in cultured for4hour group and cultured for8hours between the groups,4hours of training group and the culture differencesbetween the24hour group, there was statistically significant (P <0.05), no significantdifference between cultured for8hour group and the24hours of training group (P>0.05). V group comparison data showed that, the bacteria number was significantlyhigher in micro-arc oxidation treated group than in polished pure titanium gorup afterculture for4H,8H and24H (P <0.05).Conclusions:1The results of MTT showed that the adherenced cells on two difference surfaceswere aomost the same, which indicated that in the early stage of adhesion, micro-arcoxidation treatment and pure titanium showed the same ability in promoting theattachment of human gingival epithelial cells.2The cell proliferation cultured after1day,3day,5day,7day and theexpression of E-cad, EGF gene in the cells cultured for1day and3day detected withMTT method showed that, micro-arc oxidation treatment have some positive effects onthe early adhesion and proliferation of human gingival epithelial cells. 3With high roughness and surface energy, micro-arc oxidation treated surfacewas more conducive to the adherence of Streptococcus mutans.4According to all the above results, we think that the promotion of micro-arcoxidation treatment on the adhesion and proliferation of human gingival epithelial cellsis not strong, but its promotion of bacterial adsorption ability is strong, therefore it isnot suitable for surface treatment technology as an implant in gingival portion and abase portion. |
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