An Experimental Study Of Rose Bengal-mediated Fluorescence Spectrometry For Diagnosis Of Oral Premalignant Lesions

The early diagnosis and monitoring of high-risk individuals are important for theprevention and control of oral cancer. The most common clinical diagnosis methods,such as toluidine blue staining and brush biopsy, are simple. However, these methodsyield high false-positive rates and, thus, do not achieve the desired effect. Recently,rose bengal (RB) stain was found to readily bind with pathological tissues, effectivelydetecting the occurrence of early oral cancer lesions. In order to improve theobjectivity and practicability of RB staining, we use fluorescence spectroscopy todevelop a quantized diagnosis standard for RB-mediated diagnosis of different stagesof oral cancer and precancerous lesions and to evaluate the feasibility of this method.We detected the autofluorescence spectroscopy and the stained fluorescencespectroscopy of all four groups of animal models: group Ⅰ, Ⅱ, Ⅲ and Ⅳ. Andcalculated the diagnose ratio K, defied as K=IRB/Iauto, in which IRBpresentedfluorescence intensity of RB in tissue, and Iautopresented autofluorescence intensity.After statistical analysis, the K values among the four groups showed remarkabledifferences. The diagnostic criteria were defined as: normal, K≤8.913; simplehyperplasia, K=8.913–41.924; mild dysplasia, K=41.924–70.791; moderate andsever dysplasia, K>70.791. Compared with histopathological diagnoses, thesensitivity and specificity of this technique were both relatively high. Therefore, weconclude that RB-mediated fluorescence spectroscopy can distinguish betweennormal, hyperplasia, mild dysplasia, and moderate and severe dysplasia in thesetissues, indicating this method can be used as an important reference value for oralprecancer diagnosis.