Experimental Research Of Electro Acupuncture Intervene Rats Of Bone Ccancer Pain

Objective:By means of electroacupuncture to the bone metastasis in breast cancer, observe the effects to the pain and compare the effects of electroacupuncture to the bone metastasis in breast cancer with of drugs.Discuss the feasibility of electroacupuncture to the bone metastasis in breast cancer and get the mechanism of electroacupuncture to the bone metastasis in breast cancer to direct clinical treatment.Methods:Twenty-four female SD rats weighed180to220gram were randomly divided into three groups.They are blank group, sham-operated group and model group.Cells of Walker256were injected into the hollow pith of left hind shinbone to establish the model. The cultured cells were transferred by ascites. The models were examined by comparing with blank group and sham-operated group.forty female SD rats weighted180to220gram are divided equally into fiver groups at random.They are blank group,model group, electroacupuncture group, drug group and electroacupuncture with drug group.Observe the rats’behavior and morphology. Observe the expression of TRAP and GFAP by the means of Western-blot and Immunohistochemistry.Results:Bone damage appear in the shinbone at8days,14days,21days after inoculation by X-ray. Both hind legs’heat pain and tails’mechanical tenderness were sensitive. A lot of tumour cells were found by hematoxylin-eosin staining at left hind shinbone, after inoculated21days.After inoculated21days, Rats TRAP was higher. GFAP was activating, which is the spinal glial cell marker.Electroacupuncture, Zoledronic Acid and electroacupuncture combined with Zoledronic Acid can make the high expression of TRAP low and restrain the activating GFAP.The effect of electroacupuncture combined with Zoledronic Acid is better than only with electroacupuncture or Zoledronic Acid.Conclusion:Animal models of human breast cancer bone metastasis can be established successfully by Walker256breast cancer cells.Electroacupuncture, Zoledronic Acid and electroacupuncture combined with Zoledronic Acid can help relieve the rats’pain of breast cancer bone metastasis. The effects of electroacupuncture combined with Zoledronic Acid were better than only with electroacupuncture or Zoledronic Acid.electroacupuncture can do more effective help to the pain of cancer bone metastasis and electroacupuncture combined with drugs can increase the effects.