| Objective: To investigate roles of Coronin-1C in hepatocellular carcinoma cells, andto unclose the related mechanisms.Methods: q-PCR, Western blotting and immunochemistry were used to determine theexpression of Coronin1C mRNA and protein in HCC; HCC cell line BEL-7402cellwith stable knock-down of Coronin1C was generated. Wound healing assay and MTTassay and transwell migration assay were performed to investigate the effects ofCoronin1C on HCC cell migration, proliferation ability and invation ability respectly.Immunofluorescence and FITC-phalloidin staining metholds were used to detect thethe effect of Coronin1C on cell polarity and stress fiber network. Rac1GST-PAKpull-down assay was performed to to determine the effect of Coronin1C on Rac1activity. The effect of Coronin1C on cell tumorigenicity was evaluted by an in vivomouse model.Results: Coronin-1C was overexpressed in human HCC tissues compared with theadjacent non-tumor tissues. Overexpression of Coronin-1C enhanced the cellmigration in the human HCC cell line BEL-7402, whereas suppressed cell migrationand proliferation were observed in Coronin-1C-knockdown BEL-7402cells togetherwith impaired cell polarity, disrupted cytoskeleton and decreased Rac-1activation.Moreover, the Coronin-1C knockdown cells displayed a lower degree of malignancyby inducing smaller tumors in nude mice.Conclusion: From results mentioned above we demonstrated a relationship betweenCoronin-1C overexpression and human HCC growth through enhancement of tumorcell proliferation and migration, which are correlated with Rac-1activation. |
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