| Spinal cord ischemia-reperfusion injury as secondary injury after primary injurywas an important factor which injured the nerve cells, it referred to some factorswhich could lead removal of spinal cord ischemia and the blood supply of spinal cordrestores, but nerve function not only did not improve, but became worse on the basisof the original ischemic injury, and even the phenomenon of irreversible spinal corddelayed neuronal death. Spinal cord ischemia-reperfusion injury could causequadriplegia, paraplegia, and even death, it threaten to human health and life seriously.With further research of mechanism and basic theory of spinal cordischemia-reperfusion injury, and compared with clinical case studies, we found thatthe rate of secondary injury associated with spinal cord ischemia-reperfusion injury(such as paralysis) showed a gradual upward trend.Mechanism of spinal cord ischemia-reperfusion injury was still a hot research, the junction point of spinal cord ischemia-reperfusion injury included damage of localcirculation mechanism, apoptosis of nerve cells, ischemia (inflammation, edema)mechanism, mechanism of immune injury, the injury mechanism of the related waterchannel-like protein and various cytokines cause injury. Research on spinal cordischemia-reperfusion injury in recent years has made some progress, but the treatmentand diagnosis of spinal cord ischemia reperfusion injury has not been substantiveprogress. It’s hoped that we could find a sign of protein as the therapeutic target ofspinal cord ischemia-reperfusion injury in clinical.The nerve system of vertebrates is made of neuron and colloid, which areheterologous cellular constituents. Astrocyte can be connected in the way of mesh offibres by heterologous cellular constituents which has different shapes or functions,and maintain plasticity of cells. Among them, Vimentin and GFAP are primaryproteins.In central nerve system, the precursor of astrocyte, that is Vimentin,is themain existence form of intermediate filament protein. The maturity of astrocyte isaccompanied by transformation in Vimentin to GFAP, and the latter has been seen as amarker of the maturity of astrocyte. After the injury of CNS, the raised express ofGFAP is the main reaction of astrocyte. Therefore,not only will the correlationalresearch around the regulation of GFAP contribute to the understanding ofphysiological function of central nerve,but also play a role in neuropathology.After ischemic injury of central nerve,the expression of SPNA2mRNA which mainlly express impaired swollen cells in cortex and corpus striatum is raiseddramaticlly in penumbra.In cortex and corpus striatum of ischemic injuried centralnerve tissue and overexpansional cells in penumbra,the enhance of SPNA2expreesionindicates the early neurotic defense mechanism which involve the stimulation of nerverepair and regeneration,so,SPNA2is considered to participate in the grow of axonsand plasticity neural.In our previous research that the proteomics of acute spinal cord ischemia-reperfusion injury in rabbits,we found GFAP protein and SPNA2protein wassignificantly down-regulated expression,which showed that these proteins played animportant role in the pathological process of the spinal cord ischemia-reperfusioninjury. Through further validation of such proteins,we demonstrated the function inthe pathological changes of the spinal cord ischemia-reperfusion injury, consideringthis differential protein as a kind of iconic protein in the spinal cord ischemia-reperfusion injury,which have a great clinical significance in the pre-diagnosis,identification of the cell regulatory molecules of clinical spinal cord ischemia-reperfusion injury patients.Still having not seen such differential protein at home andabroad study.Objective:Through the further reacher of the down regulated nerve repair related proteins inspinal cord ischemia-reperfusion injury,we proved the characteristic and the mechanism of the expression changes,therefor making a solid foundation to find thenovel target of the specific diagnosis and treatment of spinal cord ischemia-reperfusion injury in clinical work.Methods:Our research has successfully established acute spinal cord ischemia-reperfusioninjury model of rabbit,and take the lumbar spinal cord of the corresponding injuredsegments as specimens,which is analyzed by DIGE technology and proteomics massspectrometry research to obtain the amount,types and changes of significant differentproteins between sham-operated control group and Ischemia-reperfusion time pointsat0,12,24,48hours.we successfully obtain the significant different proteins of eachtime point,and select the psychopathic proteins which specific down-regulated for2times by using western blotting analysis method.Results:In this paper, acute rabbit mild segmental spinal cord I/R injury animal modelwas successfully established by artery block method of L3/4/5(improved Kwunmethod).methods of proteomics two-dimensional electrophoresis analysis wasapplied to research the the injured spinal cord specimens between Sham-operatedcontrol group and reperfusion group in0,12,24and48h, combined with massspectrometry to screen for differentially expressed protein2times more than21.2kinds of Upregulated protein is analyzed through the method of WB, in all the damage time point,both protein expression in spinal cord tissue of reperfusion for24h wassignificantly lower expression, initial level gradually return to the spinal cord afterreperfusion.To research the upregulated differential protein with WB in the normalgroup and control group, the results target that the differences of protein expressionin normal group no significant change compared with different reperfusion timepoint in control of the control group differences in protein expression (P>0.05).Conclusions and innovation:We use the method of combining light microscopic histopathological andanimals in motor function segmental lumbar artery occlusion to proved that theischemia-reperfusion model preparation method targeted explicitly, the correspondingsegments of the spinal cord injury is clear, and a high success rate of I/R model,animal The mortality rate is low.Application of proteomics in combination with Western blotting, Westernblotting, WB) analysis method in the process of spinal cord ischemia-reperfusioninjury by more than2times the difference protein expression and function verificationbased on the analysis of the plot of the protein dynamics change, put forward theprocess of spinal cord ischemia-reperfusion injury in ischemia reperfusion after24hof neural function damage and repair is important point in time; Proved not puresurgery process in animal models of spinal cord tissue target protein expression hadno effect; At the protein level of spinal cord ischemia-reperfusion injury mechanism is proposed for the first time cut the expression of GFAP and SPNA2protein involved,so as to inhibit the action of the central nervous system injury repair. This experimentresearch results will provide in depth in this paper, the spinal cord ischemia-reperfusion injury in the process of the central nervous injury, repair mechanismsprovide important scientific evidence. |
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