Synthesis Of Quaternary Ammonium Salts Of Polyamide And Researches Of Silver Ion Probe Which Based On DNA

As the material base of life, DNA plays an important role in controlling a lot of biological processes. The researches of it will without a doubt help people to know about themselves in a deeper degree at molecular level. The mistake in the reproduction of DNA is closely related to the happenning of many diseases. For example, cancer is deemed to origin form gene mutation. To kill the cancer cells, we can destroy the DNA in them at the addition of drugs which will leads to the failure of DNA’s reproduction, and this is a base thinking in anti-cancer drug’s design. In addition, DNA is also widely used as a tool molecule in many fields such as medecine and materials science, especially in biological analysis due to its unique base-pairing mode and variety of secondary structures besides the use as a target in the design of anti-cancer drugs. Based on the two characters of DNA, works about the design and synthsis of drugs which target DNA as well as DNA used as a probe in the detection of silver ion were done in this thesis.(1) How to improve the selectivity of drugs is always being a big challenge in the modem anti-cancer drugs’design. To solve this problem, several strategies were developed, including the pre-drug strategy and the increase of the DNA sequence recognizing ability of drugs. Polyamide is such a molecule that has excellent DNA sequence recognizing ability. In this thesis it was designed to combine with some pre-drug molecules such as light induced DNA cross-linking agent—phenol quaternary ammonium sa Its and light induced DNA damage agent—pyrene to form some new kinds of anti-cancer drugs. Synthsis and characterization of the target molecules were done.(2) In this thesis two kinds of silver ion detection systems were designed which based on the conversion of the structure of a sequence special DNA—CatG4when reacting with sivler ion. Firstly, based on the different adsorption abilities to the fluorescent molecule—acridine orange between graphene and the G-quadruplex structure formed by CatG4, the combination and separation of acridine orange from the surface of graphene oxide could be controlled by the formation and ruin of G-quadruplex structure, A sensitively, rapidly and selectively silver ion detection system was designed and demonstrated. Secondly, as CatG4can specifically bind to silver ion which leads to the change of its structure, its combination and separation to graphene can be controlled. Fluorophores marked CatG4was used and thus to generate a change in fluorescence signal which can be used to detect silver ion. And the detection process was simplified.