| Backgroud and obiecte: Status epilepticus (SE) is a common brain insult thatmay lead to development of temporal lobe epilepsy (TLE) which is frequentlycharacterized by hippocampal sclerosis, and other areas can also be involved.However, relatively limited information is available for dynamic changes aboutbrain during disease progresssion. The present study aims to estimate thesechanges in acute, latent and chronic phases of epileptogenesis by using a longterm (until6months) MR imaging follow up in the rat pilocarpine model thatmimics most clinical and neuropathological features of TLE, and to evaluatepossible neuroprotective effect of the drugs diazepam (DZP) andPhenobarbital(PB).Materials and Methods: The study was approved by the local Animal WelfareCommittee.20female Sprague Dawley rats were studied. Status epileticus (SE)was successfully induced in14rats by administration of pilocarpine. SE wasinterrupted with either DZP and PB (n=7, group PB) or DZP alone (n=7, groupKO). MR scans were performed before (baseline), and24h,48h,1week,6weeks,3months, and6months after SE onset, to include acute and chronic phasesof epileptogenesis. MR examinations were carried out on a7Tesla animalscanner. Image J software were adapt to measure the T2relaxation time, FA value and ADC value, the region of interest(ROI) including retrosplenialparital cortex, retrosplenial temporal cortex, amygdale, piriform andenthorinal cortex, dorsal hippocampus, ventral hippocampus, posteriorhippocampus, dorsal thalamus, ventral thalamus, substantial nigra and corpuscallosum. Volumes of the hippocampus, CSF (cerebrospinal fluid) andintracranial vault were measured manually by using the free software ITK-SNAP.SPSS13.0was performed to do the Statistical analysis. The T2relaxation time,FA value and ADC value and hippocampal volume between cerebral hemispheres,different time points, and different groups were compared by using thetwo-tailed t-test.Resules: There was no significant difference in T2relaxation time, FA valueand ADC value and hippocampal volume between cerebral hemispheres. The T2relaxation time, FA value and volumes of the hippocampus, CSF and intracranialvault time dependence in different ROI showed similar in KO and PB group.However, the values of KO group changed more predominance than PB group. TheADC value time dependence appeared different between two groups.Conclusions: SE-induced neurodegeneration is a very long lasting process,which could be detected by MR T2mapping sequence, volume measurement and DTI.The combination of DZP and PB seems to have neuroprotective effects concerningbrain injury. |
