Transformation Of Epithelial Mesenchymal With Pancreatic Cancer Stem Cells Sample Correlation Studies

Introduction The epithelial-mesenchymal transition (EMT) has been linked toinduction of a stem-cell like phenotype characterized by altered cell surface markerexpression and increased tumor formation. The aim of this study was to investigate ifEMT correlates with CD24+CD44+and CD133+cells in pancreatic cancer.Methods The morphology of untreated and gemcitabine treated SW1990gemcitabine resistant cells and normal SW1990cells were compared. NF-κB p65expression was knocked down using siRNA. Vimentin and E-cadherin expressionwere analyzed using Western blot, and CD24+CD44+, CD133+cells were quantifiedby FACS.Results In SW1990gemcitabine resistant cells, gemcitabine induced a mesenchymalcell phenotype, expression of EMT-related molecμlar markers and increasedCD24+CD44+and CD133+cells compared to untreated SW1990gemcitabineresistant and SW1990cells. Knockdown NF-κB p65inhibited the ability ofgemcitabine to increase the proportion of CD24+CD44+or CD133+cells andexpression of EMT-related molecμlar markers.Conclusions This study demonstrated EMT correlated with CD24+CD44+andCD133+cells in pancreatic cancer. This study also suggested EMT may inducecancer stem like cells in pancreatic cancer, with different degrees of EMT probabilityinducing different proportions of CD24+CD44+and CD133+cells. Introduction We have obtained that epithelial mesenchymal transition correlateswith CD24+CD44+and CD133+cells in SW1990/GZ. To further validate thecorrelation also exists in the human body we conducted the next experiment.Methods Samples were obtained from41cases of pancreatic ductal adenocarcinomaconfirmed by pathological analysis. Immunohistochemistry was performed onEMT-associated markers using anti-Vimentin, anti-E-Cadherin antibodies and thestem cell-associated markers using anti-CD24and anti-CD133and CD44. The dataanalyzed by Pearson correlation test.Results Person correlative analyses of all the individual markers indicated strongpositive correlations between expression of vimentin and CD24(r2=0.8601,p<0.0001), vimentin and CD44vimentin and CD133(r2=0.6807, p<0.0001).Additionally, E-cadherin and vimentin (r2=0.2957, p=0.0002), E-cadherin and CD24(r2=0.2798, p=0.0004), E-cadherin and CD44(r2=0.3004, p=0.0002), E-cadherin andCD133(r2=0.1589, p=0.0098) expression showed significant negative correlations.Conclusions Epithelial mesenchymal transition correlates with CD24+CD44+andCD133+cells in pancreatic ductal adenocarcinoma. This study also suggested EMTmay induce cancer stem like cells in pancreatic cancer.