Dain Cow Mastitis And Intestinal Microflora Association Study

Mastitis is one of the embossed incidence diseases in the dairy cattle farm, and the traditional therapies include using of antibiotics which usually have a high recurrent rate. The disease recurrent will reduce the milk production and cause the worse results such as breast necrosis. So many cows are obsoleted because of mastitis and that cause a great economic loss to the dairy industry. Nursing mothers also suffering from mastitis and they cannot be treated with the antibiotics because the medicine have a bad influence to the baby’s nervous system. With all of the negative effects, the antibiotics cannot be used in the mastitis treatment. Therefore it is extremely urgent to develop new therapy for mastitis. Probiotics is a viable alternative which can be used in the prevention and treatment of mastitis because of many successful applications. Many previous examples in a variety of disease prevention and treatment using probiotics make this research very interesting.Digestive tract is the main niche where probiotics can play a crucial role in maintaining the host’s health. It was observed that digestive tract is one of the important microbial niche, which have a robust relationship with mastitis. This was the predominant fact of this research to use probiotic instead of antibiotics in the therapy of mastitis. So in this thesis, we designed some experiments to study the significant differences between healthy cows and the others which were subjected to mastitis. We use the real-time quantitative PCR to detect multiple bacteria genus and a number of Lactobacillus species. The results revealed L. buchneri, as the prominent species, which has the significant difference between the two groups and the contribution value is 27.32%. The second Lactobacillus species which has the largest contribution to the variation is L. sake, the value is 9.64%. We choose the germfree mouse as the adopter participate in the fecal microbiota transplantation. The feces were collected from healthy cow and mastitis cow. The germfree mice were placed into three groups, (i) the fecal microbiota from the healthy cows. (ii) fecal microbiota from the mastitis cows, (iii) the fecal microbiota from the mastitis cows which accept probiotics intervention. Meanwhile, the status of the mammary gland tissues if there has an inflammation turn up by checking the immune indexes of mammary gland tissues were checked, so that we can do the evaluation on mammary health status. The result of this experiment showed that in day 25 after fecal microbiota transplantation an obvious mammary gland inflammation turned up in the group which adopted the fecal microbiota from the mastitis cows while the situation in the probiotics intervention group is better. The group which adopted the fecal microbiota from the healthy cows did not observed obvious mammary gland inflammation. After the pathology test, we collect the feces of the three groups of mice, for further metagenomics analysis and whole length 16S rRNA gene sequencing. The result showed that there was a significant difference between the samples from cows as well as from mouse. But the group which adopted the fecal microbiota from the healthy cows has an intestinal microflora structure and function similar to healthy cows. The two groups which adopted the fecal microbiota from the mastitis cows and one of these groups which have received probiotics intervention have a different intestinal microflora structure and function when compared with the mastitis cows. Some genus have responsible for producing acetic acid, butyric acid and succinic acid have higher abundance in the healthy cows and mouses which adopted the fecal microbiota from healthy cows. By analyzing 16S rRNA gene sequencing we found that the Lactobacillus has a higher abundance in the healthy mouse. So we can see the different intestinal microflora structure and function between healthy group and mastitis group in the cows as well as in mouse. The healthy intestinal microflora structure is crucial for the cows’ health. Probiotics has the potential ability to cure mastitis in dairy cows. In addition it plays an essential role in maintaining the healthy state and mastitis prevention.