Study On Regulation And Mechanism Of Resveratrol On Immunological Stress In Chickens

In this paper, animal feeding experiments and in vitro lymphocyte culture experiment were conducted to investigate the immunomodulation and action mechanism of resveratrol(RES) on immunological stress in chickens. And from the cellular and molecular level to investigate the signal transduction mechanism of resveratrol on immunological stress in chicken by NF- κ B signal pathway.Experiment 1:To explore the Effectss and mechanism of resveratrol on growth in young chickens. 300 chicks at age of 1 day were random Ly divided into 5 groups, with 6 replicates of 10 chicks in each replicate. The negative control group was not vaccinated and fed with basal diet, RES experimental group was vaccinated once a week and fed basic diet supplemented with 0 mg/kg, 200 mg/kg, 400 mg/kg and 800 mg/kg of resveratrol. The test period was 40 days. The results showed that: ADG in the vaccinated group was significantly less than that of the control group(P < 0.05). ADG, serum IGF-1 were quadratically increased( P<0.05) with increasing RES. In the hepatocytes, GHR gene m RNA relative expression were quadratically increased and NF-κB gene m RNA relative expression was linearly decreased with increasing RES(P<0.05). In the splenocytes, IL-1β, and TNF-α m RNA relative expression were linearly decreased with increasing RES(P<0.05). For improving growth,400 mg/kg resveratrol is optimal in young chickens.Experiment 2:To explore the Effectss and mechanism of resveratrol on immune function in young chickens. Experiment design was same to Experiment 1. The results showed that: ND、H9、H5 and Ig G in the vaccinated group were significantly more than that of the control group(P < 0.05), but, CD4+/CD8+ ratio decreased significantly and thymus and bursa of Fabricius apoptosis increased significantly in the vaccinated group(P < 0.05). ND, H5 and H9 antibody titers were quadratically increased( P<0.05) with increasing RES. Quadratic improvements in the proliferation index and relative weight of thymus gland were observed with increasing RES(P<0.05). In addition, in thymocytes ligand Fas L expression was linearly decreased with increasing RES(P<0.05). For enhancing immune response,400 mg/kg resveratrol is optimal in young chickens.Experiment 3 : An immunological stress model was establish through intraperitoneal injecting with E coli lipopolysaccharide(LPS) to further evaluate the Effectss of RES on growth-depression and immune response induced by immune challenge in broilers. 120 chicks at age of 1 day were used in a 2×2 factorial design.The main factors consisted of diets(400 mg/kg RES or 0 mg/kg RES) and immunological challenge(LPS or saline). The test period was 49 days. RES supplementation decreased significantly(P<0.05) serum corticosterone and ADG increased significantly(P<0.05), feed conversion ratio decreased significantly(P<0.05) in broilers challenged by LPS. Thymus index was significantly increased(P<0.05) and thymus and liver cell apoptosis rate decreased significantly(P<0.05); SOD and T-AOC in serum increased significantly(P<0.05) and MDA decreased significantly(P<0.05). Ig G and C3 content in serum was significantly increased(P<0.05); the lymphocyte transformation rate and lymphocyte subsets and CD4/CD8 ratio increased significantly(P<0.05). Experiment 4:Regulation Effects and mechanism of resveratrol on lymphocyte apoptosis was studied in vitro. Peripheral blood lymphocytes of young chickens were challenged by LPS and were cultured with media containing various concentrations RES. RES supplementation significantly decreased the lymphocyte apoptosis rate and increased the activity of lymphocyte(P < 0.05), NF- κ B activity significantly reduced in lymphocyte culture fluid. Fas and Caspase-8 expression significantly reduced(P < 0.05). Conclusion: RES can improved growth and immune function in young chickens exprosed to immunological stress. The mechanism is closely related to inhibit the NF-κ B activation, thereby alleviating inflammatory reaction and apoptosis induced by immunological stress. The appropriate addition amount of resveratrol in the chicken’s diet is 400 mg/kg to alleviate immunological stress.