Effects Of Pichia Guilliermondii Mannan Oligosaccharide On Growth Performance And Immune Function In Pigs

Mannan oligosaccharides (MOS) derived from Saccharomyces Cerevisiae (SC) or Pichia Guilliermondii (PG). SC MOS had been demonstrated to regulate immunity and improve growth performance of animal by reducing the pathogen load in the gut. The immune regulation research of PGMOS on the stress and disease simulated condition of pigs has not been reported yet. Four experiments were conducted in this paper to investigate the effects and modulation of PGMOS on growth performance and immune function to provide a scientific basis for utilizing PGMOS in pigs.The sows were supplemented with four types of diets with the PGMOS inclusion rate of0%,0.1%,0.2%,0.4%separately. Supplementation started in late pregnancy (85d) and continued throughout lactation of28days. The results showed that birth weight, piglets weaning weight, average daily gain of suckling piglets and interval from weaning to re-mating were improved significantly with0.2%inclusion rate of PGMOS supplemented in sows. The IgG level in sows serum and colostrum were higher in0.1%,0.2%,0.4%PGMOS treatments compared with control group.One hundred and forty four weaning piglets (7.17±0.16Kg BW,28d of age) were fed with C, Tl and T2treatment diets supplemented with0%,0.1%,0.2%of PGMOS in basic diet separately for28days. The results showed the average daily gain and feed efficiency had increased in0.2%PGMOS supplements group. The intestinal villus height and the villus height/crypt depth ratio increased significantly in T1, T2groups. PGMOS treatments had a significant increase in Serum IL-10concentration and decreased serum TNF-a concentration in weaning piglets. Therefore, it suggested that PGMOS supplements could increase the weaned piglet performance by improving morphological structure of intestinal mucosa and changing the cytokine excretion in serum.Twenty-four healthy weaned piglets randomly divided into two groups and were fed in diets supplemented with0%and0.2%PGMOS separately. On d of19, all of selected pigs in each groups were injected with either LPS (100μg/kg-BW) or an equivalent amount of sterile saline. Recorded the Rectal temperature after LPS challenged. Blood samples were collected at Oh,4h,48h after LPS treatment for analysis of IL-2, IL-6, IL-10, TNF-and IFN-y in serum. The results indicated PGMOS supplements enhance the increasing of rectal temperature at4h,48post LPS challenge. The numbers of WBC increasing after LPS challenged for24h was alleviated in piglets with0.2%PGMOS treatment. The PGMOS supplements increased the concentration of IL-10post LPS challenge. It suggested that PGMOS may attenuate the immune response by increasing the anti-inflammatory cytokines (IL-10) production after LPS challenged.Twenty-four weaned crossbred barrows were randomly assigned to two treatments of Control and0.2%PGMOS groups. On d14of experiment time, half piglets of each group were challenged with30mL of E. coli K88, k99(1010cfu/mL). The growth performance, quantities of immune cells in intestinal mucosa of piglets were observed. The cytokines IL-1βp, IL-6and TLR4mRNA relative expression in intestinal mucosa were detected by Real-time PCR at d23of experiment period. The results showed that PGMOS improved the growth performance after pigs were challenged by E Coli. Intestinal intraepithelial lymphocytes cells and intraepithelial goblet cells were increased with the PGMOS treatments. The intestinal mucosa IL-1β and TLR4expression of PGMOS group were decreased in the condition without E Coli challenged. The intestinal mucosa IL-1β and IL-6mRNA expression were increased significantly of PGMOS group after challenged by E Coli. It indicated that PGMOS supplements enhanced the intestinal mucosal immunologic barrier and modulating the inflammatory with cytokines IL-1β and IL-6to improve the growth performance of weaned pigs.In conclusion, the reasons of PGMOS promoting sows reproductive and piglets growth performance is that PGMOS can improve morphological structure of intestinal mucosa and changing the cytokine excretion in serum. Furthermore, PGMOS supplements increased the anti-inflammatory cytokines and modulated the inflammatory with cytokines IL-1β, IL-6and TLR4mRNA expression to improve performance on the stress and disease simulated condition of pigs.