Study On Transformation Of Rice Salt Resistance Gene OsCYP2

Not only in China, but also over the globe, rice (Oryza Sativa L) is one of the most important crops. As most influent crops, directly rice own many things to do with national economy development and the people’s livelihood. In China, area of rice cultivation is round thirty percent of all crops, and yield of rice is around fifty percent of all others. The production system of rice plays a major role among whole national economy development.One side the population increases, another side cultivated land decreases, there is much practical value to make use of saline and alkaline land at high efficiency. To develop and cultivate high salt-tolerance plants which contain much economy and ecological wealth is one of ways for deep development of the saline and alkaline land. With progressing of modern molecular biology and gene transformation technique, a breakthrough has been made for breeding of plant, until now, there are numbers of gene which are involved with salt-tolerance already had been isolated and transferred into lots of target plants, and meantime to established a new channel to selected and developed strong salt-tolerance plant variety.In this research, salt-induced gene OsCYP2has been cloned from Japonica varieties, and transformed into rice by Agrobacterium-mediated way. The target gene expressed inside the rice, and identified with molecular methods, physiological examinations and agricultural traits investigations. Obtained multiple-generations of new rice germplasm, created a efficient system to develop new rice germplasms and new rice varieties. The outputs are as follow:(1) Isolated519base pair of salt-induced gene OsCYP2from Northern good-quality Japonica cultivar, the analytical result demonstrates the gene code172amino acids of peptide, contain cyclophilinee-ABH-like domain, belong to cyclophilin gene family,97.5%similar to whole known gene OsCYP2.(2) For detecting the function, transformed the target gene into model plant of tobacco, the consequence show the ability of salt tolerance was high improved.(3) Cultivated and selected Jinong7, Jinongda838, Jinongda1-41and others varieties of rice, finally chose Jinongda838which own more excellent agricultural traits than others material as acceptor, and then established regenerated system for Jinongda838.(4) Constructed the plant expressed vector pCAMBIA3301-OsCYPO2, transformed the target gene into rice with Agrobacterium-mediated way to over-expressed in the rice, and achieved transgenic plants.(5) Force on bar gene inside recombinant plant expressed vector, using single-prime PCR way, created a primary system to locate the foreign gene by finding out the flank sequence of inserted fragment.(6) Force on bar gene inside recombinant plant expressed vector, using single-prime PCR way, created a primary system to locate the foreign gene by finding out the flank sequence of inserted fragment.(7) Investigated a number of physiology index such as content of praline, assay of Superoxide Disemutase and others, the data showed actually the gene help rice organism to improve the resistance of salt-tolerance.(8) Characterized the resistance of salt-tolerance on transgenic rice and found the ability was precisely elevated at high level.(9) Examination on major agricultural traits of the transgenic rice, contrasted to control, nothing but reproductive period prolonged.(10) Analysis of inheritance stable ability of the rice on generations of T2and T3, figure out that target gene in progeny stably transmitted into next generation, and distribution data corresponded to Mendel’s law of inheritance.