| Based on the bio-activity collaboration between the Astragalus, Codonopsis, Tremella polysaccharide and animal gut lactobacillus, bifidus bacteria, we disigned the experiment to find a kind of biotransforation technique and apply it to develop the traditional Chinese veterinary medicine in vitro. The aim was to provide a new technology or new idea for traditional Chinese veterinary medicine innovation. The probiotics were successfully separated from Gut of chickens. The experimental probiotics were acclimated with Ultraviolet Irradiation and the herbals. The transformation model was found for herbal polysaccharides extraction with the probiotic cultivation in vitro, and successfully applied to develop a new veterinary products.(1) After being treated with the Astragalus, Codonopsis extracts and UV irradiation, Lactobacillus FGM9and streptococcus PILGM4were succesfully isolated from chicken gut.(2) The parameters of fermentation model was screenned with PILGM4proliferation and the increase rate of polysaccharide in the artificial neural network.(3) In experimental fermentation model, the results showed that the polysaccharides extraction rate in fermented product of astragalus was70.88higher than the raw Astragalus of33.53, it was82.47%higher than the raw Codonopsis of38.57%.(4) A scale-up experimental model was found. A new product including a probiotic, the fermented Astragalus and the fermented Codonopsis was produced.(5) The products were not any toxic and abnormal effects. It showed a helpful performance for body-weight gain, benefit for immunology, repairment for liver injury. The product quality could to be evaluated with the content of Calycosin-7-glucoside. |
PDF Full Text Request