Antibody Dynamic And Relevant Microrna Expression In AIV Infected SPF Ducks

MicroRNA is a non-coding small RNA with about22nucleotides in lengthendogenous that widely present in a variety of organisms. MiRNA complementarypairing with the target mRNA3′UTR region, specificity base caused the degradationof the target mRNA or inhibiting their translation in order to achieve the level oftarget gene expression regulation. It may paly important role in a variety of biologicalprocesses such as cell development, differentiation, tissues and organa formation,viral infection, et al.Avian influenza belongs to the orthomyxovirus virus family influenza virus, is apetent infectious poultry, caused by influenza A virus which can lead to human andanimal comorbid. The fact of that the incidence of death with highly pathogenic avianinfluenza virus infection waterfowl appears, human are increasingly aware of hazardsof AIV. High-throughput sequencing technology as a powerful tool for miRNAanalysis identified. Now ducks miRNA research is still in a blank stage, eapeciallyinfected and non-infected AIV duck.In this study, on the basis of collected3-month-old SPF ducks spleentissueswhich infected and non-infected H5N1subtype avian influenza virus, usinghigh-throughput sequencing analysis techniques, identification and differentiallyexpressed analysis of miRNA in infected and non-infected with the H5N1subtype ofavian influenza virus ducks spleen tissue. The miRNA has a feature of tissue-specific,it makes miRNA play a variety of different role in the process of growth anddevelopment of organisms and different tissues and organs. Further, using quantitativePCR, we analyzed the expression of some differentially expressed miRNAs in ducksvarious tissue under infected and non-infected with the H5N1subtype of avian influenza virus.The results show that we can detect the avian influenza virus expression in duckvivo after infected avian influenza virus seven days, and reaches a maximum at15days. Deep sequencing of the two libraries by using the high-throughput sequencingtechnology successfully generated. The result shown that the small RNA aresignificant more in types and quantity in SPF duck than the duck that infected H5N1subtype of avian influenza virus.322known differentially expressed miRNAs wereidentified, of which105were significantly up-regulated and217were significantlydown-regulated. In addition,17novel small RNA were identified, including1wassignificantly up-regulated miRNA and4were significantly down-regulated miRNA.The quantitative analysis of nine differentially expressed miRNA that randomlyselected, the result verified the accuracy of the results of the high-throughputsequencing.The different tissues Quantitative analysis results show that the ducks under theH5N1subtype of avian influenza virus infection, miR-17a*, miR-30c*and miR-10have highest relative expression level in muscle, miR-4006f-5p and miR-6havehighest relative expression level in trachea, miR-92b-3p has highest relativeexpression level in lungs, miR-4499highest relative expression level in spleen,miR-2808e and miR-4002-5p have highest relative expression level in heart.However，miR-17a*, miR-92b-3p, miR-30c*, miR-10and miR-6have lowest relativeexpression lever in liver. In addition, miR-4006f-5p, miR-4499and miR-2808e almosthave no relative expression in lung. In SPF ducks, miR-17a*, miR-30c*, miR-10havehighest relative expression level in lung, miR-4006f-5p has highest relative expressionlevel in muscle, miR-92b-3p and miR-4499have highest relative expression level inspleen, miR-6has highest relative expression level in kidney, miR-2808e andmiR-4002-5p have highest relative expression level in heart. However, miR-17a*,miR-4006f-5p, miR-10, miR-6and miR-4499in liver have lowest relative expressionlevel. In this study, we found that miRNA has a different expression of the same tissue,in SPF duck and infected ducks. In this study, the miRNA genomics analysis between Duck spleen tissue infectedand non-infected with the H5N1subtype of avian influenza virus were performed, theresults show that miRNA may play an important regulation role in waterfowl in therole of avian influenza virus. This result may lay the foundation for the further studyin miRNA research on the machanism of waterfowl under infected with avianinfluenza virus. At the same time provide a new theoretical basis for the developmentof disease-resistant breeding.