The Plant Print Identification And Pharmacology For Traditional Chinese Medicinal Plants Of Changbai Mountain

This study researches plant print standard samples of 236 species, 92 genera, 58 families which are based on plant print patent technique. The plant print can be classified in different styles: no-rule type, and monocotyledon with long or short cell and dumbbell pore guard cell or without long or short cell and half-moon pore guard cell, and long or short epidermis cell with half-moon pore guard cell, and special monocotyledon plant print type, such as Commelina communis Linn. and Smilacina japonica A. Gray.After the epidermis subsidiary-type, pore compound-type and lamina epidermis cell anticlinal wall-type of Changbai mountain medicinal plant have been tested, the result shows that plants have synchronism evolution characteristics in macroscopic and microcosmic system.Using correlation analysis and regression estimation, we selected some representative plants in epidermis cell anticlinal wall-type, such as Lychnis cognata Maxim, Rumex trisetifer Stokes, Eupatorium japonicum Thunb., Artemisia gmelinii Web.et Stechm., Panax ginseng C. A. Meyer, Parasenecio hastatus (Linn.) H. Koyama., to do the statistical analysis. The result shows high accuracy and reliability in plants’apparent plant print identification.Using clustering analysis, we detected six monocotyledons medicinal plants of Changbai Mountain: Echinochloa crusgali (Linn.) Beauv., Iris sanguinea Donn ex Horn, Commelina communis Linn., Typha latifolia Linn., Lilium distichum Nakai et Kamibayashi, and Smilacina japonica A. Gray. To prove system evolution law of the plant, we identified apparent plant print digitally, quantified plant print data sample distance in Euclidean space, and analyzed the paternity in plants.In this study, there’re four species of Polygonales plants: Polygonum sieboldii Meisn, Polygonum hydropiper Linn., Polygonum divaricatum Linn., Polygonum perricaria. That have been dissected and compared. The result shows that inside the mechanical tissue of Polygonum divaricatum Linn., there are six symmetrical abnormity vascular bundles, which look like monkey’s face. Regardless of the lamina, it became an inner-beginning mature structure. The same genera plants have different structures in different growing environment, which proved that plant and environment have a dialectical unity relationship. The individual development of Polygonum perricaria repeated the system development. It is the system evolution law of plant stems evolution into leaves. Unusual vascular bundles of Polygonum divaricatum Linn., show the evolution law that individual development repeats the system development.The main feature of shade environment Polygonum sieboldii Meisn, Polygonum hydropiper Linn., Polygonum divaricatum Linn., is thin and dark green lamina. It is because that they grow in the primeval forest, where thrived tall trees cover the light, making it hard to catch necessary light intensity for photosynthesis. So chloroplast macropinacoid perpendicular to leaf epidermis, dark green lamina improved the capability of plant photosynthesis in shade environment.This study also investigates the relationship between the induction of MCF-7 human breast cancer cell apoptosis by Rhizoma Paridis saponins (RPS) and the mitochondrial apoptotic pathway. We treated MCF-7 cells with RPS at various concentrations and examined the inhibitory effect of RPS on the proliferation of MCF-7 cells using the MTT assay, the change in MMP using JC-1 staining, and the expression levels of related proteins using western blot. The results showed that the inhibitory effect of RPS against the growth of MCF-7 human breast cancer cells might be related to the apoptosis induced caspase family and the caspase-3-dependent pathway. The results suggest that RPS has the potential to be a valuable anticancer agent.