| China has vast territory and abundant resources. There are kinds of microorganisms in different habitats. A survey on culturable bacterial communities in Mountain Changbai and Xinjiang was carried out. Mountain Changbai was covered with large areas of forest, while also rich in water resources and fertile soil. Xinjiang is extremely drought, rich in radiation and long-time sunshined. The unique geographical and ecological environments determined the different microbial communities.55 bacterial strains were isolated from Mountain Changbai, while 155 strains were obtained from samples in the desert of Xinjiang. Most of the isolates from Mountain Changbai were related to plant. Bacteria isolated from Xinjiang were strongly tolerance to radiation, drought and oligotrophic. The result indicated that the environments in Mountain Changbai and Xinjiang are quite different.Polyphasic taxonomic approach was used to identify four newly recovered bacterial strains.Strain 311-10T, which was isolated from a sand sample of Xinjiang in China, was characterized using a polyphasic taxonomic approach. The isolate was Gram-negative, aerobic, rod-shaped and non-motile. The major fatty acids of the strain 311-10 were :o, iso-Cn17:0 3-OH, summed feature 3 (containing C16:1ω7c and/or C16:1ω6c), summed feature 4 (comprising anteiso-C17:1 B and/or iso-C17:1 I).The predominant menaquinone of strain 311-10T was MK-7 and the genomic DNA G+C content was 47.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate formed a cluster with the genera Pontibacter and [Effluviibacter] in the phylum Bacteroidetes, with sequence similarities of 93.9-95.6%. The 16S rRNA gene sequences similarity between 311-10T and [Effluviibacter] roseus was 94.7%. The DNA G+C content of [Effluviibacter] roseus SRC-1T is 52.0-52.3 mol%. Phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analysis support the reclassification of [Effluviibacter] roseus as Pontibacter roseus comb. nov. (type strain SRC-1T=MTCC 7260T=DSM 17521T) and proposal a novel species, Pontibacter xinjiangensis sp. nov., with strain 311-10 (=CCTCC AB 207200T=NRRL B-51335 T) as the type strain.Two novel strains, designated HY-50RT and HY-49MT, were both Gram-negative and strictly aerobic. Isolate HY-50RT can grow on NA, R2A and TSA, but not on MacConkey. Colonies on 0.1×TSA were circular, smooth and yellow. Cells were rod-shaped. The polyamine pattern showed a predominance of homospermidine. The predominant menaquinone of strain HY-50RT was MK-7 and the genomic DNA G+C content was 46.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate was a member of the phylum Bacteroidetes, with the lower similarities with its closest relatives. The highest similarity between HY-50RT and Niastella populi THYL-44T was 93.6%. The major fatty acids were iso-C15:1 G (11.7 %), iso-C15:0 (19.6%) and iso-C17:0 3-OH (19.3%). On the basis of the polyphasic data from this study, the isolate represents a novel genus and species, for which the name Flavitalea populi gen. nov., sp. nov. was proposed. The type strain is HY-50RT (=CCTCC AB 208255T=NRRL B-59222T).Isolate HY-49MT was rod-shaped and motile by single polar. Colonies on 0.3×MA were circular, convex, smooth, opaque and white. Can grow on MA and R2A, but not on TSA, NA or MacConkey. Q-10 was the predominant respiratory ubiquinone. The genomic DNA G+C content was 60.4 mol%. The major fatty acids were C16:0, C18:0,11-methyl C18;1ω7c, C19:0ω8c cyclo and summed feature 8(C18:1ω7c或C18: 1ω6c). The isolate was clustered with the genus Pelagibacterium in the phylogenetic tree constructed on the basis of 16S rRNA gene sequences. It showed some similar pattern of biochemical and physiological features with the genus Pelagibacterium. These results convinced that strain HY-49MT belongs to the genus Pelagibacterium. However, the isolate showed sufficient phenotypic, phylogenetic and genetic differentiations from known Pelagibacterium species. In all, strain HY-49MT should represent a novel species of the genus Pelagibacterium, for which the name Pelagibacterium luojinesis was proposed. The type strain is HY-49MT (=CCTCC AB 208257T).Another bacterium obtained from soil of Tibet in China was characterized using a polyphasic taxonomic approach. Strain XM415T was aerobic, Gram-negative, gliding and rod-shaped. Ubiquinone Q-8 and branched fatty acids, such as iso-C16:o (24.0%), iso-C15:0 (22.6%), iso-C17:1ω9c (6.7%), iso-C14:0(6.1%) and iso-C11:0 3OH (5.2%) predominantly appeared in strain XM415 as well as in all type strains of the recognized Lysobacter species. The DNA G+C content of XM415T was 63.5 mol%. Strain XM415 showed the highest similarity of 16S rRNA gene sequence with Lysobacter niastensis DSM 18481T (96.0%). The genotypic and phenotypic data show that strain XM415T merits classification as representing a novel species of the genus Lysobacter, for which the name Lysobacter ximonensis sp. nov. is proposed. The type strain is XM415T(=CCTCC AB 20709T=NRRL B-51263T). |
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