Experimental And Clinical Study Of Xiongbing Spray In Treating Acute Cerebral Ischemic Stroke

Objective:To investigate the effect and mechanism of Xiongbing spray in treating acute cerebral ischemic stroke by experimental and clinical study, objective evaluate the effect of nanometer praeparatum by comparing to the common praeparatum, and provide the bases for using Xiongbing spray in clinical practice.Method:Animal experimental study:seventy-eight SD rats were randomly divided into six groups:the pseudo operated group, the model group (operated), Nimoldipine group, Common praeparatum group, and nanometer praeparatum groups (treated with high and low dose respective). The rats cerebral ischemic stroke model were subjected to focal ischemic by middle cerebral artery occlusion(MCAO)using intraluminal thread. The neurologic impairment scoring, cerebral ischemic area measured by TTC-staining, the quantity of gene expression by Caspase-3 and AQP4 in ischemic brain tissue, the result of pathology photograph, and the level of TNF-αand IL-1βin the peripheral blood were observed and compared between groups.clinical study:sixty patients were randomly divided into therapy group (treated with Xiongbing spray and general westen medicine) and controlled group(only using general westen medicine), the treatment period was two weeks. The neurologic impairment scoring, clinical effect of TCM syndrome were observed before and after treatment, as well as the security of Xiongbing spray.Result:Animal experimental study:Common praeparatum, nanometer praeparatum with high dose and nimoldipine could ameliorate the scorings of neural deficit of cerebral ischemic stroke rats, reduce cerebral ischemic area measured by TTC-staining, decrease quantity of gene expression by Caspase-3 and AQP4 in ischemic brain tissue, active suppress the increase of TNF-αand IL-1βin the peripheral blood, significant reductions of nerval tissue damage were also found in the three groups by pathology photograph. Experimental result show that therapeutic effect of three kinds of praeparatum above-mentioned is similar on the whole, while part of it manifest common praeparatum and high dose nanometer praeparatum surpass nimoldipine.clinical study:the ameliorations of the scoring of neural deficit in Xiongbing spray group were predominant compared with controlled group, the same case could be seen in the scoring of TCM syndrome while curative effect of TCM syndrome without statistics difference between groups. The function of liver and renal, blood general measuring were normal before and after treatment,there was no severe adverse reactions in patients caused by Xiongbing spray.Conclusions:Xiongbing spray has remarkable protective effect against focal brain damage induced by occlusion-reperfusion, which can inhibiting neural cells apotosis, reduce ischemic brain edema and controling Inflammatory reaction. nanometer praeparatum has its own predominance although need to improve. Xiongbing spray could ameliorate the scorings of neural deficit in patients, We can conclude that Xiongbing spray be one of ideal medicines for acute cerebral ischemic stroke.