| Objective:The inhibitive effects of superparamagnetic paclitaxel nanoparticles to the malignant glioma growth were studied both in vivo and in vitro.Methods:Superparamagnetic paclitaxel nanoparticles were fabricated by nanotechnology before their diameters, magnetism as well as drug release rate were tested. After incubation with human malignant glioma cell line U251, the intracellular distribution of nanoparticles was observed. Afterwards the cytotoxicity and apoptosis-inducing capacity of superparamagnetic paclitaxel nanoparticles at different concentrations to U251 were tested by CCK-8 kit and flow cytometry. Rat malignant glioma cell line C6 was implanted into SD rats' head stereotactically to obtain the glioma-bearing rats. After intravenous administration of superparamagnetic paclitaxel nanoparticles and magnetic targeting, the rats'survival was recorded and compared with that after pure paclitaxel injection.Results:Superparamagnetic paclitaxel nanoparticles had a mean diameter of 20 nm with a narrow distribution. They were superparamagnetic and released drugs for more than 15 days constantly. Besides they could enter the glioma cells effectively and inducing apoptosis with a similar cytotoxicity with paclitaxel. After intravenous injection of superparamagnetic paclitaxel nanoparticles and magnetic targeting with a 0.5T magnet, the survival of glioma-bearing rats were prolonged for 1 fold compared to rats treated with paclitaxel.Conclusion:Superparamagnetic paclitaxel nanoparticles converted the poor solubility of paclitaxel. They are safe and stable with a long release of loaded drugs. The superparamagnetic paclitaxel nanoparticles can enter the glioma cells to inhibit their growth because of their ultrasmall size. Besides, they can accumulate in magnetic targeted area and result in a high local drug concentration to inhibit the tumor growth and prolong the glioma-bearing rats'survival. |
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