Studies On The Curing Of Nasopharyngealcarcinoma (NPC) With Officinal Dendrobium Stem

Purpose Now the main metheds curing with nasopharyngeal carcinoma include radiotherapy and chemotherapy,both of those have severe side effect, and the long distance diversion survival rate for five years is about 70%～80%,so seanching more metheds for NPC therapy is a hot topic. Officinal Dendrobium Stem belongs to the entire grass with perennial attachment herb,witch is full of polysaccharide,alkaloid,as well as necessary microelement and amino acids etc. the Extraction from officinal Dendrobium Stem can suppress liver cancer and Osteosarcoma in mice,and also can prevent the resistance of e multi-medicines in tumor. But until now, there are any report with the role of anti-nasopharyngeal carcinoma of officinal Dendrobium Stem.The purpose of our study is to research the effect of officinal Dendrobium Stem on CNE1 and CNE2 cells,tring to find its molecular mechanismsPart oneStudies on the Inhibiting nasopharyngealcarcinoma Effect of Officinal Dendrobium Stem and the serum including Officinal Dendrobium Stem in vitroMethods The cells of CNE1 and CNE2 are interfered by Officinal Dendrobium Stem and the serum including Officinal Dendrobium Stem. Then the growth inhibition and apoptosis of CNEl and CNE2 cells and the protein levels of Bcl-xL, mcl-1,caspase-3,caspase-8 and caspase-9 were examined by MTT assay, lightmicroscopy, flow cytometry and Western blot, respectively.Results MTT assay showed that 6000 CNE1 or CNE2 cells in one hole in 96 orifices were the best growing. It suggest that both of the extractive of fresh Officinal Dendrobium Stem and the serum with Officinal Dendrobium Stem, significantly inhibited proliferation of CNE1 and CNE2 cells Officinal Dendrobium Stem has the higher inhibition ratio.flow cytometry analysis showed:compared to the control group, both of the extractive of fresh Officinal Dendrobium Stem and the serum with Officinal Dendrobium Stem played an Obvious role in promoting apoptosis to CNE1 and CNE2 cells(P<0.01). The result was Closely Related with time and density.The detect of flow cytometry revealed:the cycle blockage of the four concentration gradient of Officinal Dendrobium Stem took place at the stage of G0/G1.The cell proportion of this period was higher than the control group(p<0.01),and obviously lower than control group at the stage of G2/M(p<0.01).The cyclomorphosis effect of serum with Officinal Dendrobium Stem of the four concentration gradient on the two NPC cell strain,.For the CNE1, all cycle blockage happened at the stage of G0/G1 excepting the concentration of 10%.For the CNE2,all cycle blockage happened at the stage of G0/G1 excepting the concentration of 15%. The cell proportion of this period was higher than the control group(p<0.01), and obviously lower than control group at the stage of G2/M(p<0.01).Western blot showed:both of the Bcl-xl and Mcl-1 expression were decreased. Caspase-8 and caspase-9 expression were expressed fully, specially for the group of 256mg/mL. The caspase-3 expression was also expressed. Part twoThe effect of Officinal Dendrobium Stem in Null Mice Grafted with Nasopharyngeal CarcinomaMethed The CNE1 and CNE2 cells were grafted in the null mice,when the tumor growed to a certain volume, the null mice were divided into control group and medicine group, were investigated by HE staining.Ultra micro-structured changes of Carcinoma cells were observed by transmission electron microscope(TEM).In TUNEL of histochemistry were used to detect the apoptosis of Carcinoma cells.Immunohistochemical method were used to detect the expression of Bcl-xL, mcl-1 and caspase-3.Results The volume and the the weigh of tumor wrer inhibited by Officinal Dendrobium Stem. The tumor cells were decreased and fibrous tissue was increased in the Medication group. Under ultramicrostructure apoptotic body can be seen. The rate of cell apoptosis for the Officinal Dendrobium Stem groups were higher than that of the control.The expression of Bcl-xL and mcl-1 and were lower and the expression of caspase-3 were higher than that of the control.Conclusion Officinal Dendrobium Stem was an effective Traditional Chinese medicine to cure of nasopharyngealcarcinoma witch Promoting to apoptotic and Suppressing the muLtiplication. It also could inhibite the growth the Nasopharyngeal Carcinoma witch is grafted in the null mice. This might relay with a diminution of the expression of the anti-apoptotic protein Mcl-land Bcl-xL.Also may be related with the way of caspase. This may indicatethat that both of the mitochondrial pathway and the death pathway were clearly involved.